Difference between revisions of "Part:BBa M50005:Experience"

 
Line 5: Line 5:
  
 
===Applications of BBa_M50005===
 
===Applications of BBa_M50005===
 +
BBa_M50005 contains the alkG protein (ABO_2708) originating in the bacterium Alcanivorax borkumensis. It is meant to be used in conjunction with BBa_M50004 (alkB1 protein, ABO_2708) and other enzymes from A. borkumensis to degrade alkanes measuring up to 32 carbons as part of A. borkumensis' digestive process.
  
 
===User Reviews===
 
===User Reviews===
 +
<I>Stanford BioE44 "Team WERK"</I>
 +
<BR>We transformed E. coli with both BBa_M50004 and BBa_M50005 in an attempt to replicate the first step of A. borkumensis' alkane degradation in different bacteria. Under proper induction (rhamnose), our Western blot protocol revealed high levels of alkG production. However, pH testing failed to confirm the cooperative function of alkB1 and alkG, with transformed bacteria in alkane-rich conditions behaving relatively indistinguishably from those in standard conditions. This may have been due to a failure in double transformation, with the two proteins being expressed at disproportionate levels. <BR><BR>
 +
 
<!-- DON'T DELETE --><partinfo>BBa_M50005 StartReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_M50005 StartReviews</partinfo>
 
<!-- Template for a user review
 
<!-- Template for a user review
−
{|width='80%' style='border:1px solid gray'
+
<!-- {|width='80%' style='border:1px solid gray'
−
|-
+
<!-- |-
−
|width='10%'|
+
<!-- |width='10%'|
−
<partinfo>BBa_M50005 AddReview number</partinfo>
+
<!-- <partinfo>BBa_M50005 AddReview number</partinfo>
−
<I>Username</I>
+
<!-- <I>Username</I>
−
|width='60%' valign='top'|
+
<!-- |width='60%' valign='top'|
−
Enter the review inofrmation here.
+
<!-- Enter the review inofrmation here.
−
|};
+
<!-- |};
 
<!-- End of the user review template -->
 
<!-- End of the user review template -->
 
<!-- DON'T DELETE --><partinfo>BBa_M50005 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_M50005 EndReviews</partinfo>
 +
 +
===Stanford Location===
 +
Plasmid name: ABO-2708 <BR>DNA2.0 Gene #: pD861-CH<BR>Organism: E. coli<BR>Device type: Actuator<BR>Glycerol stock barcode: 0133021780<BR>Box label: BioE44 F16

Revision as of 05:02, 11 December 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_M50005

BBa_M50005 contains the alkG protein (ABO_2708) originating in the bacterium Alcanivorax borkumensis. It is meant to be used in conjunction with BBa_M50004 (alkB1 protein, ABO_2708) and other enzymes from A. borkumensis to degrade alkanes measuring up to 32 carbons as part of A. borkumensis' digestive process.

User Reviews

Stanford BioE44 "Team WERK"
We transformed E. coli with both BBa_M50004 and BBa_M50005 in an attempt to replicate the first step of A. borkumensis' alkane degradation in different bacteria. Under proper induction (rhamnose), our Western blot protocol revealed high levels of alkG production. However, pH testing failed to confirm the cooperative function of alkB1 and alkG, with transformed bacteria in alkane-rich conditions behaving relatively indistinguishably from those in standard conditions. This may have been due to a failure in double transformation, with the two proteins being expressed at disproportionate levels.

UNIQa5d5846f79b275a2-partinfo-00000000-QINU UNIQa5d5846f79b275a2-partinfo-00000001-QINU

Stanford Location

Plasmid name: ABO-2708
DNA2.0 Gene #: pD861-CH
Organism: E. coli
Device type: Actuator
Glycerol stock barcode: 0133021780
Box label: BioE44 F16