Difference between revisions of "Part:BBa K1955003"

 
 
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<b style="font-size:23px;">pSB1C3-5'HYG</b><br><br>
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We replaced p36 and nagt genes with a cloning site for an exogenous gene and with hyg as a selection marker, respectively. This dual-function biobrick enables stable expression of foreign proteins by drug selection in Leishmania.<br><br>
  
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__NOTOC__
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<b style="font-size:20px;">(1) The basic part checked by PCR: </b>
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<partinfo>BBa_K1955003 short</partinfo>
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The coding sequence of Hemagglutinin (HA) from H1N1 acquired from NCBI. HA is a glycoprotein found on the surface of influenza viruses and can be used in immunological experiments as the target antigen.
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We used pSB1C3-5’HYG, pSB1C3-3’UTR, pSB1C3-HA, pSB1C3-OVA as template, to check the length of the inserts. The PCR reaction was performed with Taq polymerase, and screened in 0.8％ agarose gel by electrophoresis.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 08:12, 5 December 2016

pSB1C3-5'HYG

We replaced p36 and nagt genes with a cloning site for an exogenous gene and with hyg as a selection marker, respectively. This dual-function biobrick enables stable expression of foreign proteins by drug selection in Leishmania.

(1) The basic part checked by PCR:

We used pSB1C3-5’HYG, pSB1C3-3’UTR, pSB1C3-HA, pSB1C3-OVA as template, to check the length of the inserts. The PCR reaction was performed with Taq polymerase, and screened in 0.8％ agarose gel by electrophoresis.





Sequence and Features BBa_K1955003 SequenceAndFeatures