Difference between revisions of "Part:BBa K2110200"

Latest revision as of 13:21, 16 November 2016

PETase expression system in Saccharomyces cerevisiae

Saccharomyces cerevisiae is a widely used chassis organism in synthetic biology research. We use it as the chassis organism to express the PETase gene and assay the enzyme activity. This composite part consists of the enhanced promoter PGK1 (BBa_K2110012), α signal peptide (BBa_K2110017), PETase gene (or its various mutants, BBa_K2110800, BBa_K2110100-BBa_K2110109), and CYC1 terminator (BBa_K2110000). The expression data can be accessed respectively in the part information page of PETase gene and its mutants. (BBa_K2110800, BBa_K2110100-BBa_K2110109).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1141
Illegal BglII site found at 1351
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2110200 short</partinfo>
-Saccharomyces cerevisiae is a widely used chassis organism in synthetic biology research. We use it as the chassis organism to express the PETase gene and assay the enzyme activity. This composite part consists of the enhanced promoter PGK1 (BBa_K2110012), &#945; signal peptide (BBa_K2110017), PETase gene (or its various mutants, BBa_K2110800, BBa_K2110100-BBa_K2110109), and CYC1 terminator (BBa_K2110001). The expression data can be accessed respectively in the part information page of PETase gene and its mutants. (BBa_K2110800, BBa_K2110100-BBa_K2110109).
+Saccharomyces cerevisiae is a widely used chassis organism in synthetic biology research. We use it as the chassis organism to express the PETase gene and assay the enzyme activity. This composite part consists of the enhanced promoter PGK1 (BBa_K2110012), &#945; signal peptide (BBa_K2110017), PETase gene (or its various mutants, BBa_K2110800, BBa_K2110100-BBa_K2110109), and CYC1 terminator (BBa_K2110000). The expression data can be accessed respectively in the part information page of PETase gene and its mutants. (BBa_K2110800, BBa_K2110100-BBa_K2110109).
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