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| | <partinfo>BBa_K2012009 short</partinfo> | | <partinfo>BBa_K2012009 short</partinfo> |
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| − | Bacteria movement related gene CheZ which fused with a GS linker and GFP in C teminal. We will use this part to charactise the expression of CheZ.
| + | <span style="font-style:italic"><span style="font-style:normal"><span style="font-style:italic">cheZ</span> gene, </span><span style="font-style:normal">regulating the bacterial motility by decreasing the possibility of tumbling,</span></span><span> is </span><span></span><span></span><span></span><span></span><span></span><span></span><span>a chemotaxis relating gene</span><span style="font-style:italic"><span style="font-style:normal">, It is wildly used in iGEM to control the motility of bacteria. In igem 2016 HZAU-China's project, we successfully fused a reporter protein GFP(BBa_E0040) with CheZ through a 18bp GS linker to monitor the concentration of CheZ.<br/> |
| − | <html> | + | We have proved the function of emitting green light under certain exciting light and the function of improving motility by transform it into a <span style="font-style:italic">cheZ</span> lacking strain, CL1. </span></span> |
| − | <div>
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| − | <div style="height:auto!important;margin-left:auto;margin-right:auto;min-height:595.3pt;padding-bottom:1pt;padding-top:1pt">
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| − | <div style="margin-left:auto;margin-right:auto;position:relative"><p style="text-align:center"><span style="font-family:宋体;font-size:18.0pt">户口迁移登记表</span><span style="font-family:Times New Roman;font-size:9.0pt"><span></span></span></p> | + | |
| − | <p style="text-align:center"><span style="font-family:Times New Roman;font-size:9.0pt"> </span></p> | + | |
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| − | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | + | <div> <span style="color:rgb(40, 40, 40);font-family:arial, sans-serif;font-size:13px">These parts are present in plasmid pSB1C3, but there is also a constitutive promoter (J23100-derived) inserted into the XbaI site. So, for example, the EcoRI/PstI region of part K2012009 reads:</span><br/> |
| − | <tbody><tr> <td style="border-bottom:solid windowtext 1.0pt;border-left:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;border-top:solid windowtext 1.0pt;padding:0pt 5.4pt 0pt 5.4pt" valign="top"> <p style="text-align:center"><span style="font-family:宋体;font-size:14.0pt">姓名<span></span></span></p>
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| − | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;border-top:solid windowtext 1.0pt;padding:0pt 5.4pt 0pt 5.4pt" valign="top"> <p style="text-align:center"><span style="font-family:宋体;font-size:14.0pt">专业<span></span></span></p>
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| − | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;border-top:solid windowtext 1.0pt;padding:0pt 5.4pt 0pt 5.4pt" valign="top"> <p style="text-align:center"><span style="font-family:宋体;font-size:14.0pt">身份证号<span></span></span></p>
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| − | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;border-top:solid windowtext 1.0pt;padding:0pt 5.4pt 0pt 5.4pt" valign="top"> <p style="text-align:center"><span style="font-family:宋体">市内迁入</span><span style="font-family:Times New Roman"><span></span></span></p>
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| − | <p style="text-align:center"><span style="font-family:Times New Roman">(打√)</span><span style="font-family:Times New Roman"><span></span></span></p>
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| − | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;border-top:solid windowtext 1.0pt;padding:0pt 5.4pt 0pt 5.4pt" valign="top"> <p style="text-align:center"><span style="font-family:宋体">市外迁入</span><span style="font-family:Times New Roman"><span></span></span></p>
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| − | <p style="text-align:center"><span style="font-family:Times New Roman">(打√)</span><span style="font-family:Times New Roman"><span></span></span></p>
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| − | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;border-top:solid windowtext 1.0pt;padding:0pt 5.4pt 0pt 5.4pt" valign="top"> <p style="text-align:center"><span style="font-family:宋体;font-size:14.0pt">签名<span></span></span></p>
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| − | </td> <td style="border-bottom:solid windowtext 1.0pt;border-left:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;border-top:solid windowtext 1.0pt;padding:0pt 5.4pt 0pt 5.4pt" valign="top"> <p style="text-align:center"><span style="font-family:宋体;font-size:14.0pt">备注<span></span></span></p>
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| − | </td> </tr></tbody>
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| − | </table>
| + | |
| − | <p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:宋体;font-size:14.0pt">此表格</span><span style="font-family:宋体;font-size:14.0pt;font-weight:bold">纸质版</span><span style="font-family:宋体;font-size:14.0pt">与</span><span style="font-family:宋体;font-size:14.0pt;font-weight:bold">电子版</span><span style="font-family:宋体;font-size:14.0pt">均要上交。如有疑问，可咨询保卫处户籍室：87282107。<span></span></span></p>
| + | |
| − | <p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:宋体;font-size:14.0pt">此表格需加盖学院公章。<span> </span>辅导员签字：<span> </span>联系电话：</span><span style="font-family:Times New Roman;font-size:14.0pt"><span></span></span></p>
| + | |
| | </div> | | </div> |
| − | </div>
| + | <div ><span>------------------------------------------------------------------------------------------------------------------------------------------------</span><br/> |
| − | <br/> | + | Biobrick 5' XbaI J23100 XbaI <span style="font-weight:bold"> K2012009 </span> Biobrick 3' <br/> |
| − | </div> | + | gaattcgcggccgcttctagaGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTtctaga<b><span style="font-weight:normal">...................................</span></b><span>actagtagcggccgctgcag</span> |
| − | | + | --------------------------------------------------------------------------------------------------------------------------------------------------------------------- </div> |
| | + | <p style="margin-top:0.4em;margin-bottom:0.5em;color:rgb(40, 40, 40);font-family:arial, sans-serif;font-size:13px">This feature in no way prevents the use of these parts in standard Biobrick assembly. Normal prefix insertion into EcoRI/XbaI will delete this promoter element. Suffix insertion into SpeI/PstI will retain this promoter, but it can of course be removed later by a prefix insertion.</p> |
| | + | <p style="margin-top:0.4em;margin-bottom:0.5em;color:rgb(40, 40, 40);font-family:arial, sans-serif;font-size:13px">Note also that the base 5' to the SpeI site is allowed to float in these parts and is therefore rarely "T". The "G" downstream of the XbaI site obeys the standard. Because the database does not permit variation at this position, the predicted sequences of composite parts derived from these parts will be incorrect at this position.(some content are cited from J61100 part([https://parts.igem.org/Part:BBa_K819009 BBa_J61100]))</p> |
| | + | <html> |
| | + | </br> |
| | + | </br> |
| | + | <img src="https://static.igem.org/mediawiki/parts/9/97/T--HZAU--China--light_field_of_BBa_K2012009.png" style="width:400px;margin-left:5%;"/> |
| | + | <img src="https://static.igem.org/mediawiki/parts/a/a5/T--HZAU--China--fluorescence_field_of_BBa_K2012009.png" style="width:400px;margin-right:5%;"/> |
| | + | <p><b>Figure 1.</b> <i>E. coli</i> with BBa_K2012009 was observed under light field. <b>Figure 2.</b> <i>E. coli</i> with BBa_K2012009 was observed under fluorescence field.</p> |
| | + | </br> |
| | + | </br> |
| | + | </br> |
| | </html> | | </html> |
| − |
| |
| | <!-- Add more about the biology of this part here | | <!-- Add more about the biology of this part here |
| | ===Usage and Biology=== | | ===Usage and Biology=== |
This feature in no way prevents the use of these parts in standard Biobrick assembly. Normal prefix insertion into EcoRI/XbaI will delete this promoter element. Suffix insertion into SpeI/PstI will retain this promoter, but it can of course be removed later by a prefix insertion.
Note also that the base 5' to the SpeI site is allowed to float in these parts and is therefore rarely "T". The "G" downstream of the XbaI site obeys the standard. Because the database does not permit variation at this position, the predicted sequences of composite parts derived from these parts will be incorrect at this position.(some content are cited from J61100 part(BBa_J61100))
