Difference between revisions of "Part:BBa K2012007"

Revision as of 15:17, 1 November 2016 (view source) HZAU BGMa (Talk | contribs) m ← Older edit		Latest revision as of 15:19, 1 November 2016 (view source) MinTT (Talk | contribs)
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	<img src="https://static.igem.org/mediawiki/parts/f/f7/T--HZAU-China--J23101-cheZ-GFP.jpg" style="width:600px;margin-left:5%;"/>		<img src="https://static.igem.org/mediawiki/parts/f/f7/T--HZAU-China--J23101-cheZ-GFP.jpg" style="width:600px;margin-left:5%;"/>
−	<p><b>Figure 1.</b> <i>E.coli</i> with a generator containing our fragment was observed under light field and fluorescence field.</p>	+	<p><b>Figure 1.</b> <i>E. coli</i> with a generator containing our fragment was observed under light field and fluorescence field.</p>
−	<img src="https://static.igem.org/mediawiki/parts/c/cb/T--HZAU-China--CheZ-GFP_swarming.jpg" style="width:600px;margin-left:5%;"/>	+
−	<p><b>Figure 2.</b> The swarming plate of <i>E.coli</i> K12 cheZ lacing strain strain with different strength expression of cheZ-GFP.</p>	+
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−	We've also constructed a series of generators with different strength promoter,BBa_K2012013[https://parts.igem.org/Part:BBa_K2012013], BBa_K2012014[https://parts.igem.org/Part:BBa_K2012014],BBa_K2012016[https://parts.igem.org/Part:BBa_K2012016]
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		+	<img src="https://static.igem.org/mediawiki/parts/c/cb/T--HZAU-China--CheZ-GFP_swarming.jpg" style="width:600px;margin-left:5%;"/>
		+	<p><b>Figure 2.</b> The swarming plate of <i>E. coli</i> K12 cheZ lacing strain strain with different strength expression of cheZ-GFP.</p>
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−		+	We've also constructed a series of generators with different strength promoter,BBa_K2012013[https://parts.igem.org/Part:BBa_K2012013], BBa_K2012014[https://parts.igem.org/Part:BBa_K2012014],BBa_K2012016[https://parts.igem.org/Part:BBa_K2012016]
	<!-- Add more about the biology of this part here		<!-- Add more about the biology of this part here
	===Usage and Biology===		===Usage and Biology===

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Latest revision as of 15:19, 1 November 2016

CheZ fussed with a CG linker and reporter GFP(BBa_E0040)

The whole part contains CheZ fused with GFP,which can show the concentration of CheZ. This coding part do not have its RBS and promoter. Users have to add their preferred RBS and promoter by using biobrick assembly or other suitable assembly. We have proved the function of emitting green light under certain exciting light and the function of improving motility by transform it into a cheZ lacking strain, CL1.  

Figure 1. E. coli with a generator containing our fragment was observed under light field and fluorescence field.

Figure 2. The swarming plate of E. coli K12 cheZ lacing strain strain with different strength expression of cheZ-GFP.

We've also constructed a series of generators with different strength promoter,BBa_K2012013[1], BBa_K2012014[2],BBa_K2012016[3] Sequence and Features