Difference between revisions of "Part:BBa K2012007"
 
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<partinfo>BBa_K2012007 short</partinfo>
 
<partinfo>BBa_K2012007 short</partinfo>
  
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the whole part contains CheZ fused with GFP,which can show the concentration of CheZ. This coding part do not have its RBS and promoter. Users have to add their preferred RBS and promoter by using biobrick assembly or other suitable assembly.<\br>
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The whole part contains CheZ fused with GFP,which can show the concentration of CheZ. This coding part do not have its RBS and promoter. Users have to add their preferred RBS and promoter by using biobrick assembly or other suitable assembly.
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<p><span style="font-style:italic"><span style="font-style:normal"><span style="font-style:italic">cheZ</span> gene,&nbsp;</span><span style="font-style:normal">regulating the bacterial motility by &nbsp;decreasing the possibility of tumbling,</span></span><span>&nbsp;is&nbsp;</span><span></span><span></span><span></span><span></span><span></span><span></span><span>a chemotaxis relating gene</span><span style="font-style:italic"><span style="font-style:normal">, It is wildly used in iGEM to control the motility of bacteria.&nbsp;In igem 2016&nbsp;HZAU-China's project,&nbsp;we successfully fused&nbsp;a reporter protein GFP(BBa_E0040) with CheZ through a 18bp GS linker&nbsp;to monitor the concentration of CheZ.<br/>
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We have proved the function of emitting green light under certain exciting light and the function of improving motility by transform it into a <span style="font-style:italic">cheZ</span> lacking strain, CL1.&nbsp;&nbsp;
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We have proved the function of emitting green light under certain exciting light and the function of improving motility by transform it into a <span style="font-style:italic">cheZ</span> lacking strain, CL1.&nbsp;&nbsp;</span></span>
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<img src="https://static.igem.org/mediawiki/parts/f/f7/T--HZAU-China--J23101-cheZ-GFP.jpg" style="width:600px;margin-left:5%;"/>
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<p><b>Figure 1.</b> <i>E. coli</i> with a generator containing our fragment was observed under light field and fluorescence field.</p>
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<img src="https://static.igem.org/mediawiki/parts/c/cb/T--HZAU-China--CheZ-GFP_swarming.jpg" style="width:600px;margin-left:5%;"/>
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<p><b>Figure 2.</b> The swarming plate of <i>E. coli</i> K12 cheZ lacing strain strain with different strength expression of cheZ-GFP.</p>
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We've also constructed a series of generators with different strength promoter,BBa_K2012013[https://parts.igem.org/Part:BBa_K2012013], BBa_K2012014[https://parts.igem.org/Part:BBa_K2012014],BBa_K2012016[https://parts.igem.org/Part:BBa_K2012016]
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 15:19, 1 November 2016


CheZ fussed with a CG linker and reporter GFP(BBa_E0040)

The whole part contains CheZ fused with GFP,which can show the concentration of CheZ. This coding part do not have its RBS and promoter. Users have to add their preferred RBS and promoter by using biobrick assembly or other suitable assembly. We have proved the function of emitting green light under certain exciting light and the function of improving motility by transform it into a cheZ lacking strain, CL1.  


Figure 1. E. coli with a generator containing our fragment was observed under light field and fluorescence field.



Figure 2. The swarming plate of E. coli K12 cheZ lacing strain strain with different strength expression of cheZ-GFP.



We've also constructed a series of generators with different strength promoter,BBa_K2012013[1], BBa_K2012014[2],BBa_K2012016[3] Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1301