Difference between revisions of "Part:BBa K2012014"
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This generator contains promoter J23106 ,RBS from BBa_J61100,functional chemotaxis gene CheZ,reporter gene GFP from BBa_E0040 and a 18bp-GS linker between two functional domains.It can generate medium concentration of CheZ which can be reported by the fluorescence intensity.It is used to detect the relationship of CheZ under different strength of promoter and its swimming ability.Promoters with different strength are also constructed as BBa_K2012013[https://parts.igem.org/Part:BBa_K2012013],BBa_K2012016[https://parts.igem.org/Part:BBa_K2012016]
 
This generator contains promoter J23106 ,RBS from BBa_J61100,functional chemotaxis gene CheZ,reporter gene GFP from BBa_E0040 and a 18bp-GS linker between two functional domains.It can generate medium concentration of CheZ which can be reported by the fluorescence intensity.It is used to detect the relationship of CheZ under different strength of promoter and its swimming ability.Promoters with different strength are also constructed as BBa_K2012013[https://parts.igem.org/Part:BBa_K2012013],BBa_K2012016[https://parts.igem.org/Part:BBa_K2012016]
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<p>We have proved the function of GFP and CheZ by observing it under the fluorescence microscope and transforming it into a cheZ lacking strain to process swarming assay seperately<br/> the image are as following </p>
 
 
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<p>We have proved the function of GFP and CheZ by observing it under the fluorescence microscope and transforming it into a cheZ lacking strain to process swarming assay seperately.</p>
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<p>The image are as following.</p>
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</br></br>
 
<img src="https://static.igem.org/mediawiki/parts/7/7b/T--HZAU-China--CL1_control.jpg" style="width:600px;margin-left:5%;"/>
 
<img src="https://static.igem.org/mediawiki/parts/7/7b/T--HZAU-China--CL1_control.jpg" style="width:600px;margin-left:5%;"/>
 
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<p>Figure 1.<i>CL1 control was observed under light field and fluorescence field</p>.       
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<p><b>Figure 1.</b> CL1 control was observed under light field and fluorescence field.</p>.</br></br>      
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<img src="https://static.igem.org/mediawiki/parts/3/3d/T--HZAU-China--J23106-cheZ-GFP.jpg" style="width:700px;margin-left:0%;"/>
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<img src="https://static.igem.org/mediawiki/parts/f/f7/T--HZAU-China--J23101-cheZ-GFP.jpg" style="width:600px;margin-left:5%;"/>
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<p><b>Figure 2.</b> <i>E.coli</i> with BBa_K2012013 was observed under light field and fluorescence field.</p></br></br>
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<p>Figure 2.<i>E.coli</i> with BBa_K2012014 was observed under light field and fluorescence field;</p>
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<img src="https://static.igem.org/mediawiki/parts/c/cb/T--HZAU-China--CheZ-GFP_swarming.jpg" style="width:600px;margin-left:5%;"/>
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<html><img src="https://static.igem.org/mediawiki/parts/c/cb/T--HZAU-China--CheZ-GFP_swarming.jpg" style="width:600px;margin-left:5%;"/>
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<p><b>Figure 3.</b> The swarming plate of <i>E.coli</i> K12 cheZ lacing strain strain with different strength expression of cheZ-GFP.</p>
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<p>Figure 3. The swarming plate of <i>E.coli</i> K12 cheZ lacing strain strain with different strength expression of cheZ-GFP </p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 15:12, 1 November 2016


A medium CheZ generator with fusion GFP reporter

This generator contains promoter J23106 ,RBS from BBa_J61100,functional chemotaxis gene CheZ,reporter gene GFP from BBa_E0040 and a 18bp-GS linker between two functional domains.It can generate medium concentration of CheZ which can be reported by the fluorescence intensity.It is used to detect the relationship of CheZ under different strength of promoter and its swimming ability.Promoters with different strength are also constructed as BBa_K2012013[1],BBa_K2012016[2]

We have proved the function of GFP and CheZ by observing it under the fluorescence microscope and transforming it into a cheZ lacking strain to process swarming assay seperately.

The image are as following.




Figure 1. CL1 control was observed under light field and fluorescence field.

.

Figure 2. E.coli with BBa_K2012013 was observed under light field and fluorescence field.



Figure 3. The swarming plate of E.coli K12 cheZ lacing strain strain with different strength expression of cheZ-GFP.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1362