Difference between revisions of "Part:BBa K2012013"
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<p>We have proved the function of GFP and CheZ by observing it under the fluorescence microscope and transforming it into a cheZ lacking strain to process swarming assay seperately.</p> | <p>We have proved the function of GFP and CheZ by observing it under the fluorescence microscope and transforming it into a cheZ lacking strain to process swarming assay seperately.</p> | ||
<p>The image are as following.</p> | <p>The image are as following.</p> | ||
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<img src="https://static.igem.org/mediawiki/parts/7/7b/T--HZAU-China--CL1_control.jpg" style="width:600px;margin-left:5%;"/> | <img src="https://static.igem.org/mediawiki/parts/7/7b/T--HZAU-China--CL1_control.jpg" style="width:600px;margin-left:5%;"/> | ||
</br> | </br> | ||
− | <p><b>Figure 1.</b> CL1 control was observed under light field and fluorescence field.</p>. | + | <p><b>Figure 1.</b> CL1 control was observed under light field and fluorescence field.</p>.</br></br> |
<img src="https://static.igem.org/mediawiki/parts/f/f7/T--HZAU-China--J23101-cheZ-GFP.jpg" style="width:600px;margin-left:5%;"/> | <img src="https://static.igem.org/mediawiki/parts/f/f7/T--HZAU-China--J23101-cheZ-GFP.jpg" style="width:600px;margin-left:5%;"/> | ||
− | <p><b>Figure 2.</b> <i>E.coli</i> with BBa_K2012013 was observed under light field and fluorescence field.</p> | + | <p><b>Figure 2.</b> <i>E.coli</i> with BBa_K2012013 was observed under light field and fluorescence field.</p></br></br> |
<img src="https://static.igem.org/mediawiki/parts/c/cb/T--HZAU-China--CheZ-GFP_swarming.jpg" style="width:600px;margin-left:5%;"/> | <img src="https://static.igem.org/mediawiki/parts/c/cb/T--HZAU-China--CheZ-GFP_swarming.jpg" style="width:600px;margin-left:5%;"/> | ||
<p><b>Figure 3.</b> The swarming plate of <i>E.coli</i> K12 cheZ lacing strain strain with different strength expression of cheZ-GFP.</p> | <p><b>Figure 3.</b> The swarming plate of <i>E.coli</i> K12 cheZ lacing strain strain with different strength expression of cheZ-GFP.</p> | ||
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</html> | </html> | ||
Latest revision as of 15:08, 1 November 2016
Strong CheZ generator with a fusion GFP reporter
This generator contains promoter J23101 ,RBS from BBa_J61100,functionaal chemotaxis gene CheZ,reporter gene GFP from BBa_E0040 and a 18bp-GS linker between two functional domains. Promoters with different strength are also constructed as BBa_K2012014[1],BBa_K2012016[2]
We have proved the function of GFP and CheZ by observing it under the fluorescence microscope and transforming it into a cheZ lacking strain to process swarming assay seperately.
The image are as following.
Figure 1. CL1 control was observed under light field and fluorescence field.
.Figure 2. E.coli with BBa_K2012013 was observed under light field and fluorescence field.
Figure 3. The swarming plate of E.coli K12 cheZ lacing strain strain with different strength expression of cheZ-GFP.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 715
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1362