Difference between revisions of "Part:BBa K2012013"

Revision as of 15:06, 1 November 2016

Strong CheZ generator with a fusion GFP reporter

This generator contains promoter J23101 ,RBS from BBa_J61100,functionaal chemotaxis gene CheZ,reporter gene GFP from BBa_E0040 and a 18bp-GS linker between two functional domains. Promoters with different strength are also constructed as BBa_K2012014[1],BBa_K2012016[2]

We have proved the function of GFP and CheZ by observing it under the fluorescence microscope and transforming it into a cheZ lacking strain to process swarming assay seperately.

The image are as following.

Figure 1. CL1 control was observed under light field and fluorescence field.

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Figure 2. E.coli with BBa_K2012013 was observed under light field and fluorescence field.

Figure 3. The swarming plate of E.coli K12 cheZ lacing strain strain with different strength expression of cheZ-GFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 715
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1362

@@ Line 10: / Line 10: @@
 <img src="https://static.igem.org/mediawiki/parts/7/7b/T--HZAU-China--CL1_control.jpg" style="width:600px;margin-left:5%;"/>
 </br>
-<p><b>Figure 1.<b> CL1 control was observed under light field and fluorescence field.</p>.
+<p><b>Figure 1.</b> CL1 control was observed under light field and fluorescence field.</p>.
 <img src="https://static.igem.org/mediawiki/parts/f/f7/T--HZAU-China--J23101-cheZ-GFP.jpg" style="width:600px;margin-left:5%;"/>
 <p><b>Figure 2.</b> <i>E.coli</i> with BBa_K2012013 was observed under light field and fluorescence field.</p>