Difference between revisions of "Part:BBa K2012017"
m
 
(5 intermediate revisions by 3 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2012017 short</partinfo>
 
<partinfo>BBa_K2012017 short</partinfo>
  
−
this generator is altered from BBa_K819010[https://parts.igem.org/Part:BBa_K819010] created by Peking University.Its previous promoter , J23117,is replaced by a much stronger promotor J23101. This generator is constructed to detect the relationship between different promoters' strength  with their swarming ability. A series of Biobricks were constructed as BBa_K2012018[https://parts.igem.org/Part:BBa_K2012018], BBa_K2012020[https://parts.igem.org/Part:BBa_K2012020]
+
 
−
<html><img src="https://static.igem.org/mediawiki/parts/7/7b/T--HZAU-China--CL1_control.jpg" style="width:600px;margin-left:5%;"/></html>
+
This generator is altered from BBa_K819010[https://parts.igem.org/Part:BBa_K819010] created by Peking University.Its previous promoter , J23113,is replaced by a much stronger promotor J23101. This generator is constructed to detect the relationship between different promoters' strength  with their swarming ability. A series of Biobricks were constructed as BBa_K2012018[https://parts.igem.org/Part:BBa_K2012018], BBa_K2012020[https://parts.igem.org/Part:BBa_K2012020]
−
<p>The swarming plate of different <i>E.coli</i> K12 cheZ lacking strain, CL1, transformed with our constructed plamids</p>
+
<html>
 +
</br>
 +
</br>
 +
</br>
 +
<img src="https://static.igem.org/mediawiki/parts/0/00/T--HZAU-China--swarming_plate_of_CheZ.jpg" style="width:800px;margin-left:0%;"/>
 +
<p><b>Figure 1.</b> The swarming plate of different <i>E.coli</i> K12 cheZ lacking strain, CL1, transformed with our constructed plamids.</p>
 +
</html>
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:47, 1 November 2016


A strong generator of CheZ


This generator is altered from BBa_K819010[1] created by Peking University.Its previous promoter , J23113,is replaced by a much stronger promotor J23101. This generator is constructed to detect the relationship between different promoters' strength with their swarming ability. A series of Biobricks were constructed as BBa_K2012018[2], BBa_K2012020[3]


Figure 1. The swarming plate of different E.coli K12 cheZ lacking strain, CL1, transformed with our constructed plamids.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]