Difference between revisions of "Part:BBa K2145105:Experience"
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===Applications of BBa_K2145105=== | ===Applications of BBa_K2145105=== | ||
[[File:Splates95102.png]]<br> | [[File:Splates95102.png]]<br> | ||
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+ | Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see GG98 above). Fluorescence was observed even without the addition of inducers (IPTG and ATc), even though the reporters should be under IPTG and ATc control. We attribute this to insufficient repression by LacI and TetR. | ||
[[File:Alverno_ca_98.png]] | [[File:Alverno_ca_98.png]] |
Revision as of 00:16, 31 October 2016
Applications of BBa_K2145105
Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see GG98 above). Fluorescence was observed even without the addition of inducers (IPTG and ATc), even though the reporters should be under IPTG and ATc control. We attribute this to insufficient repression by LacI and TetR.
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UNIQb6d87ae6c3c515c4-partinfo-00000000-QINU UNIQb6d87ae6c3c515c4-partinfo-00000001-QINU