Difference between revisions of "Part:BBa K2145001:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR> | This experience page is provided so that any user may enter their experience using this part.<BR> | ||
| − | Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see | + | Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see GG96 above). Fluorescence was observed even without the addition of inducers (IPTG and ATc), even though the reporters should be under IPTG and ATc control. We attribute this to insufficient repression by LacI and TetR. |
===Applications of BBa_K2145001=== | ===Applications of BBa_K2145001=== | ||
Revision as of 00:00, 31 October 2016
This experience page is provided so that any user may enter their experience using this part.
Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see GG96 above). Fluorescence was observed even without the addition of inducers (IPTG and ATc), even though the reporters should be under IPTG and ATc control. We attribute this to insufficient repression by LacI and TetR.
Applications of BBa_K2145001
User Reviews
UNIQf1e5e788b7895a6c-partinfo-00000000-QINU UNIQf1e5e788b7895a6c-partinfo-00000001-QINU