Difference between revisions of "Part:BBa K2145000"

Revision as of 22:56, 30 October 2016

GFP/RFP reporter (Forward/Forward) with random 500bp spacer

This part contains two fluorescent protein expression units (for RFP and GFP) with promoters, RBSs, CDSs, and terminators, both facing forward, with a random 500bp spacer between the two reporters. Parts were amplified from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled with golden gate assembly to make the full part. This composite part is designed to demonstrate the effect, if any, of a 500 bp spacer between genes on supercoiling generated by the two genes, and therefore on interference between the genes.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2226
Illegal AgeI site found at 2338
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 724

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 <partinfo>BBa_K2145000 short</partinfo>
-Reporter plasmid to demonstrate the effect of a random 500 bp spacer on reporter expression. This part contains two fluorescent protein coding sites (for RFP and GFP), both facing forward, and was assembled using Golden Gate Assembly. To make the plasmid, parts were amplified from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part.
+This part contains two fluorescent protein expression units (for RFP and GFP) with promoters, RBSs, CDSs, and terminators, both facing forward, with a random 500bp spacer between the two reporters. Parts were amplified from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled with golden gate assembly to make the full part. This composite part is designed to demonstrate the effect, if any, of a 500 bp spacer between genes on supercoiling generated by the two genes, and therefore on interference between the genes.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
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 <span class='h3bb'>Sequence and Features</span>