Difference between revisions of "Part:BBa I746201"

Latest revision as of 11:49, 13 October 2007

Outer Membrane Permeability Device

This device employs the FepA L8T mutant described here to allow molecules larger than normal to pass through the outer membrane of E. coli. The porins usually present will allow molecules up to around 700Da to freely diffuse in and out, however with this device, molecules of at least 1400Da should be able to pass through. Permeability was tested using the antibiotic Bacitracin. Wild type E. coli are resistant to this as the molecule is 1422Da in size. Expressing the device made the bacteria sensitive to bacitracin. In this device, the mutant FepA is under the control of a relatively high strength promotor.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 299
Illegal AgeI site found at 665
Illegal AgeI site found at 1292
Illegal AgeI site found at 1810
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_I746201 short</partinfo>
-This device employs the FepA L8T mutant described [https://parts.igem.org/wiki/index.php?title=Part:BBa_I746200 here]
+This device employs the FepA L8T mutant described [https://parts.igem.org/wiki/index.php?title=Part:BBa_I746200 here] to allow molecules larger than normal to pass through the outer membrane of E. coli. The porins usually present will allow molecules up to around 700Da to freely diffuse in and out, however with this device, molecules of at least 1400Da should be able to pass through. Permeability was tested using the antibiotic Bacitracin. Wild type E. coli are resistant to this as the molecule is 1422Da in size. Expressing the device made the bacteria sensitive to bacitracin. In this device, the mutant FepA is under the control of a relatively high strength promotor.
-<!-- Add more about the biology of this part here to allow molecules larger than normal to pass through the outer membrane of E. coli. The porins usually present will allow molecules up to around 700Da to freely diffuse in and out, however with this device, molecules of at least 1400Da should be able to pass through. Permeability was tested using the antibiotic Bacitracin. Wild type E. coli are resistant to this as the molecule is 1422Da in size. Expressing the device made the bacteria sensitive to bacitracin. In this device, the mutant FepA is under the control of a relatively high strength promotor.
 ===Usage and Biology===