Difference between revisions of "Part:BBa K1961005"
 
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<partinfo>BBa_K1961005 short</partinfo>
 
<partinfo>BBa_K1961005 short</partinfo>
  
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PbrR is a Pb<sup>2+</sup> binding protein.  In addition to the PbrR generator with the P<sub>Tet</sub> promoter(K1961004), we designed a PbrR generator (K1961005; also with GFP reporter) under control of general promoter(J23102).  We compared their GFP expression    to measure their PbrR generation.
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PbrR is a Pb<sup>2+</sup> binding protein.  In addition to the PbrR generator with the P<sub>Tet</sub> promoter(K1961004), we designed a PbrR generator (K1961005; also with GFP reporter) under control of general promoter(J23102).   
 
   
 
   
 
[[File:HSiTAIWAN_WIKI_K1961005_auto_circuit_1.jpeg|400px|thumb|left|alt text]]
 
[[File:HSiTAIWAN_WIKI_K1961005_auto_circuit_1.jpeg|400px|thumb|left|alt text]]
  
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We compared their GFP expressio to measure their PbrR generation.
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We tested the survival of E.coli in tetracycline(TC).  E. coli can survival in TC of 1000 ng/ml.  In the following figure, it shows that GFP expression of autoregulation(BBa_K1961004) is slower than that with constitutive promoter(BBa_K1961005)
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[[File: HSiTAIWAN_Autoregulation_Table.jpeg|600px|thumb|left|alt text]]
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[[File:HSiTAIWAN_Electronic.jpeg|600px|thumb|left|alt text]]
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 20:44, 30 October 2016


PbrR generator expressed by GFP under control of general promoter (J23102)

PbrR is a Pb2+ binding protein. In addition to the PbrR generator with the PTet promoter(K1961004), we designed a PbrR generator (K1961005; also with GFP reporter) under control of general promoter(J23102).

alt text

We compared their GFP expressio to measure their PbrR generation.

We tested the survival of E.coli in tetracycline(TC). E. coli can survival in TC of 1000 ng/ml. In the following figure, it shows that GFP expression of autoregulation(BBa_K1961004) is slower than that with constitutive promoter(BBa_K1961005)

alt text
alt text

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1111