Difference between revisions of "Part:BBa K1961001"
 
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The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility.  When DNA is damaged and forms single-stranded DNA, Rec A binds single-stranded DNA (ssDNA).  The binding of RecA onto ssDNA forms  a nucleoprotein filament that promotes LexA cleavage (thereby inducing the SOS response).  Recombinase mediator proteins RecF, RecO, and RecR act together to promote loading of RecA onto single stranded DNA.   
 
The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility.  When DNA is damaged and forms single-stranded DNA, Rec A binds single-stranded DNA (ssDNA).  The binding of RecA onto ssDNA forms  a nucleoprotein filament that promotes LexA cleavage (thereby inducing the SOS response).  Recombinase mediator proteins RecF, RecO, and RecR act together to promote loading of RecA onto single stranded DNA.   
  
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[[File:HSiTAIWAN_Electronic.jpeg|600px|thumb|left|alt text]]
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'''Background '''<br>
 
'''Background '''<br>
  
 
RecA is a protein in nearly all bacteria and all organisms. Following a break on a single-stranded DNA (ssDNA), the exonuclease RecJ cleaves single-stranded DNA. The single-strand DNA-binding protein (SSB) binds to the daughter strand gap, preserving the integrity of the DNA. Recombinase mediator proteins RecF, RecO, and RecR act together to promote loading of RecA onto single stranded DNA. Rec A binds single-stranded DNA (ssDNA), forming a nucleoprotein filament that promotes LexA cleavage (thereby inducing the SOS response).
 
RecA is a protein in nearly all bacteria and all organisms. Following a break on a single-stranded DNA (ssDNA), the exonuclease RecJ cleaves single-stranded DNA. The single-strand DNA-binding protein (SSB) binds to the daughter strand gap, preserving the integrity of the DNA. Recombinase mediator proteins RecF, RecO, and RecR act together to promote loading of RecA onto single stranded DNA. Rec A binds single-stranded DNA (ssDNA), forming a nucleoprotein filament that promotes LexA cleavage (thereby inducing the SOS response).
  
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[[File:pbrr1.png]]
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[[File:The FOR pathway and RecA filament in the process of SOS response.jpeg|400px|thumb|left|alt text]]
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[[File:The FOR pathway and RecA filament in the process of SOS response.jpeg|200px|thumb|left|alt text]]
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This figure is obtained from the reference below.
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'''Refernce '''<br>
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Naofumi Handa, Katsumi Morimatsu, Susan T. Lovett, Stephen C. Kowalczykowski. Reconstitution of initial steps of dsDNA break repair by the RecF pathway of E. coli. GENES & DEVELOPMENT (2009). 23(10):1234–1245
 
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===Background ===
 
===Background ===
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https://static.igem.org/mediawiki/2016/0/0c/HSiTAIWAN_PROJECT_AFLATOXIN_Figure_3_The_FOR_pathway_and_RecA_filament_in_the_process_of_SOS_response.jpeg
 
https://static.igem.org/mediawiki/2016/0/0c/HSiTAIWAN_PROJECT_AFLATOXIN_Figure_3_The_FOR_pathway_and_RecA_filament_in_the_process_of_SOS_response.jpeg
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https://parts.igem.org/File:HSiTAIWAN_Electronic.jpeg
  
 
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Latest revision as of 20:42, 30 October 2016


RecF protein-SOS mediator protein

The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. When DNA is damaged and forms single-stranded DNA, Rec A binds single-stranded DNA (ssDNA). The binding of RecA onto ssDNA forms a nucleoprotein filament that promotes LexA cleavage (thereby inducing the SOS response). Recombinase mediator proteins RecF, RecO, and RecR act together to promote loading of RecA onto single stranded DNA.

alt text

Background

RecA is a protein in nearly all bacteria and all organisms. Following a break on a single-stranded DNA (ssDNA), the exonuclease RecJ cleaves single-stranded DNA. The single-strand DNA-binding protein (SSB) binds to the daughter strand gap, preserving the integrity of the DNA. Recombinase mediator proteins RecF, RecO, and RecR act together to promote loading of RecA onto single stranded DNA. Rec A binds single-stranded DNA (ssDNA), forming a nucleoprotein filament that promotes LexA cleavage (thereby inducing the SOS response).


alt text

This figure is obtained from the reference below.

Refernce
Naofumi Handa, Katsumi Morimatsu, Susan T. Lovett, Stephen C. Kowalczykowski. Reconstitution of initial steps of dsDNA break repair by the RecF pathway of E. coli. GENES & DEVELOPMENT (2009). 23(10):1234–1245 Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 229
    Illegal SapI.rc site found at 395