Difference between revisions of "Part:BBa K1961005"
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===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 20:02, 30 October 2016


PbrR generator expressed by GFP under control of general promoter (J23102)

PbrR is a Pb2+ binding protein. In addition to the PbrR generator with the PTet promoter(K1961004), we designed a PbrR generator (K1961005; also with GFP reporter) under control of general promoter(J23102). We compared their GFP expression to measure their PbrR generation.

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We tested the survival of E.coli in tetracycline(TC). E. coli can survival in TC of 1000 ng/ml. In the following figure, it shows that GFP expression of autoregulation(BBa_K1961004) is slower than that with constitutive promoter(BBa_K1961005)

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Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1111