Difference between revisions of "Part:BBa K1996000"

Putative promoter for suspected ethylene-oxidising genes sourced from Mycobacterium NBB4 ===Usage and Biology=== This part was obtained from the ethylene metabolism operon in Mycobacterium NBB4. It is thought to be a promoter that regulates bidirectional transcription of gene involved in ethylene metabolism (Figure 1). The enzymes required for ethylene metabolism are contained within an operon organised in two bidirectional gene clusters. One gene cluster encodes the catabolic enzymes required to convert ethylene into acetyl-CoA, the final product of the degradation pathway which is subsequently directed to the tricarboxylic acid cycle. The other gene cluster encodes the enzymes responsible for synthesising coenzyme M, a cofactor required for the function of the second enzyme in the metabolic pathway.

EtnR1 binding to EtnP DNA has been demonstrated in an electrophoretic mobility shift assay (EMSA), where it was found that EtnR1 binding to EtnP notably inhibits the migration of the DNA through the gel, indicating a strong binding affinity between EtnR1 protein and EtnP DNA (Figure 2).