Difference between revisions of "Part:BBa K2070010:Design"

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===Design Notes===
 
===Design Notes===
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The part sequence was designed with reference to the amnio acid sequence provided by Rhodius V.A et al (2014). Specific sequences were selected so that no illegal cut would be introduced and so that parts could be synthesised by IDT gene fragments.
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===Source===
 
===Source===
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===References===
 
===References===
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Rhodius, V. A., Segall-Shapiro, T. H., Sharon, B. D., Ghodasara, A., Orlova, E., Tabakh, H., Voigt, C. A. (2014, 04). Design of orthogonal genetic switches based on a crosstalk map of  s, anti- s, and promoters. Molecular Systems Biology, 9(1), 702-702. doi:10.1038/msb.2013.58

Latest revision as of 16:06, 30 October 2016


ecf antisigma35


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part sequence was designed with reference to the amnio acid sequence provided by Rhodius V.A et al (2014). Specific sequences were selected so that no illegal cut would be introduced and so that parts could be synthesised by IDT gene fragments.

Source

Pseudomonas aeruginosa PAO1

References

Rhodius, V. A., Segall-Shapiro, T. H., Sharon, B. D., Ghodasara, A., Orlova, E., Tabakh, H., Voigt, C. A. (2014, 04). Design of orthogonal genetic switches based on a crosstalk map of s, anti- s, and promoters. Molecular Systems Biology, 9(1), 702-702. doi:10.1038/msb.2013.58