Difference between revisions of "Part:BBa K1916002"
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A GAF domain of the cyanobacteriochrome (CBCR) Cyan7427_1390 with an intein/chitin-binding domain attached to the C-terminus for purification. As with most CBCRs, NpF requires a phycocyanobilin co-factor, and must therefore be expressed in a system with heme oxygenase (BBa_I15008) and PcyA (BBa_I15009).
 
A GAF domain of the cyanobacteriochrome (CBCR) Cyan7427_1390 with an intein/chitin-binding domain attached to the C-terminus for purification. As with most CBCRs, NpF requires a phycocyanobilin co-factor, and must therefore be expressed in a system with heme oxygenase (BBa_I15008) and PcyA (BBa_I15009).
 +
[[Image:K1916102_cyan_spectra.jpeg|600px|thumb|left|Fig.1 Absorbance spectra of the Cyan protein extracted from the cells. Peak absorbance occurs around 695nm.]]
  
 
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Revision as of 14:39, 30 October 2016


Cyan7427_1390g3 + intein-CBD tag

A GAF domain of the cyanobacteriochrome (CBCR) Cyan7427_1390 with an intein/chitin-binding domain attached to the C-terminus for purification. As with most CBCRs, NpF requires a phycocyanobilin co-factor, and must therefore be expressed in a system with heme oxygenase (BBa_I15008) and PcyA (BBa_I15009).

Fig.1 Absorbance spectra of the Cyan protein extracted from the cells. Peak absorbance occurs around 695nm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1096
    Illegal AgeI site found at 1186
  • 1000
    COMPATIBLE WITH RFC[1000]