Difference between revisions of "Part:BBa I730011"
 
Line 2: Line 2:
 
<partinfo>BBa_I730011 short</partinfo>
 
<partinfo>BBa_I730011 short</partinfo>
  
−
pOmpR-pTetR-strong RBS-LacI repressor(LVA+)-strong RBS-YFP-terminator
+
pOmpR-pTetR-strong RBS-LacI repressor(LVA+)-strong RBS-YFP(AAV+)-terminator
  
 
R0082.R0040.B0034.C0012.E0434  
 
R0082.R0040.B0034.C0012.E0434  

Latest revision as of 16:37, 11 October 2007

pOmpR-ptetR-LacI repressor-YFP

pOmpR-pTetR-strong RBS-LacI repressor(LVA+)-strong RBS-YFP(AAV+)-terminator

R0082.R0040.B0034.C0012.E0434

intermediate part for the construction of our 'comparator'. note that it bears no terminator sequence, as we aim to add a fluorescence protein as multicistronic parts.

it has the osmolarity-sensing promoter for the OmpC gene, as the input of the device.

as an output we have thought of the fluorescence protein YFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1343
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]