Difference between revisions of "Part:BBa I730011"
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<partinfo>BBa_I730011 short</partinfo> | <partinfo>BBa_I730011 short</partinfo> | ||
| − | pOmpR-pTetR-strong RBS-LacI repressor(LVA+)-strong RBS-YFP-terminator | + | pOmpR-pTetR-strong RBS-LacI repressor(LVA+)-strong RBS-YFP(AAV+)-terminator |
R0082.R0040.B0034.C0012.E0434 | R0082.R0040.B0034.C0012.E0434 | ||
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it has the osmolarity-sensing promoter for the OmpC gene, as the input of the device. | it has the osmolarity-sensing promoter for the OmpC gene, as the input of the device. | ||
| − | as an output we have thought of the fluorescence protein | + | as an output we have thought of the fluorescence protein YFP. |
Latest revision as of 16:37, 11 October 2007
pOmpR-ptetR-LacI repressor-YFP
pOmpR-pTetR-strong RBS-LacI repressor(LVA+)-strong RBS-YFP(AAV+)-terminator
R0082.R0040.B0034.C0012.E0434
intermediate part for the construction of our 'comparator'. note that it bears no terminator sequence, as we aim to add a fluorescence protein as multicistronic parts.
it has the osmolarity-sensing promoter for the OmpC gene, as the input of the device.
as an output we have thought of the fluorescence protein YFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1343
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]