Difference between revisions of "Part:BBa K2070007:Design"

Latest revision as of 10:33, 30 October 2016

Toehold Trigger Inducible GFP generator

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 692
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 709

Design Notes

There is no TACTAGAG sequence between toehold RBS sequence and GFP sequense.

Source

Assembly.

References

Alexander A. Green, Pamela A. Silver, James J. Collins, and Peng Yin, (2014, 11) Toehold Switches: De-Novo-Designed Regulators of Gene Expression. Cell 159, 925–939.

Revision as of 10:28, 30 October 2016 (view source) Nasu (Talk \| contribs) (→‎Source) ← Older edit		Latest revision as of 10:33, 30 October 2016 (view source) Nasu (Talk \| contribs) (→‎References)
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	===References===		===References===
		+	Alexander A. Green, Pamela A. Silver, James J. Collins, and Peng Yin, (2014, 11) Toehold Switches: De-Novo-Designed Regulators of Gene Expression. Cell 159, 925–939.