Difference between revisions of "Part:BBa K2165004:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The | + | The [http://rulai.cshl.edu/cgi-bin/SCPD/getgene2?CUP1 SCPD] was used to determine whether or not the removal of an internal XbaI site was feasible. Base pair number 106 was originally a guanine and was substituted with an adenine in the 5' to 3' direction to eliminate the XbaI site in the middle of the sequence. This site is one of two Heat Shock Protein binding site, and as such the usage of any assay related to cell stress will likely be less effective with this promoter. A larger Heat Shock Protein binding site remains in tact, but heat shock proteins bind cooperatively. |
===Source=== | ===Source=== | ||
Revision as of 03:59, 30 October 2016
CUP1 yeast inducible promoter with RFC[10] restriction sites removed
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The [http://rulai.cshl.edu/cgi-bin/SCPD/getgene2?CUP1 SCPD] was used to determine whether or not the removal of an internal XbaI site was feasible. Base pair number 106 was originally a guanine and was substituted with an adenine in the 5' to 3' direction to eliminate the XbaI site in the middle of the sequence. This site is one of two Heat Shock Protein binding site, and as such the usage of any assay related to cell stress will likely be less effective with this promoter. A larger Heat Shock Protein binding site remains in tact, but heat shock proteins bind cooperatively.
Source
The CUP1 promoter was identified in the 5' untranslated region of the metallothionein gene in Saccharomyces cerevisiae.