Difference between revisions of "Part:BBa K2139002"

Revision as of 03:05, 30 October 2016

Coding Sequence of Gluc1C

BBa_K2139002 encompass the coding region for an 1,4-beta-glucosidase known as Gluc1C. Gluc1C is a monomeric enzyme capable of catalyzing the removal of decomposition from cellobiose down to glucose. It can be used in conjunction with an exo-glucanase and endo-beta-1,4-glucanase for the conversion of cellulose to glucose. The genbank ascension number for this protein is JQ713769.1 (DNA), AFQ36783.1 (amino acid), and a PDB code of 2O9R_A. This construct was synthesized for use in Caulobacter crescentus and is codon optimized to contain a high GC content.

Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 264
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1138

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 <partinfo>BBa_K2139002 short</partinfo>
-BBa_K2139002 encompass the coding region for an 1,4-beta-glucosidase known as Gluc1C. Gluc1C is a monomeric enzyme capable of catalyzing the removal of cellobiose from the end of the cellulose polymer. It can be used in conjunction with an exoglucanase for the direct conversion of cellulose into monomeric glucose. The genbank ascension number for this protein is JQ713769.1 (DNA), AFQ36783.1 (amino acid), and a PDB code of 2O9R_A. This construct was synthesized for use in Caulobacter crescentus and is codon optimized to contain a high GC content.
+BBa_K2139002 encompass the coding region for an 1,4-beta-glucosidase known as Gluc1C. Gluc1C is a monomeric enzyme capable of catalyzing the removal of decomposition from cellobiose down to glucose. It can be used in conjunction with an exo-glucanase and endo-beta-1,4-glucanase for the conversion of cellulose to glucose. The genbank ascension number for this protein is JQ713769.1 (DNA), AFQ36783.1 (amino acid), and a PDB code of 2O9R_A. This construct was synthesized for use in Caulobacter crescentus and is codon optimized to contain a high GC content.
 Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154