Difference between revisions of "Part:BBa K2139003"
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<partinfo>BBa_K2139003 short</partinfo> | <partinfo>BBa_K2139003 short</partinfo> | ||
| − | BBa_K2139003 encompass the coding region for an | + | BBa_K2139003 encompass the coding region for an 1,4-beta-glucanase known as E1. E1 catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exoglucanases for the direct conversion of cellulose into monomeric glucose. The polymerization of the protein is monomeric with an optimal temperature and pH over a wide range (unpublished data). The genbank ascension number for this protein is WP_011719450.1 (amino acid) and a PDB code of 1ECE. This construct was codon optimized for E. coli. |
Literature data for this part can be found http://aem.asm.org/content/76/19/6360.full | Literature data for this part can be found http://aem.asm.org/content/76/19/6360.full | ||
Revision as of 03:03, 30 October 2016
Coding Sequence of E1
BBa_K2139003 encompass the coding region for an 1,4-beta-glucanase known as E1. E1 catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exoglucanases for the direct conversion of cellulose into monomeric glucose. The polymerization of the protein is monomeric with an optimal temperature and pH over a wide range (unpublished data). The genbank ascension number for this protein is WP_011719450.1 (amino acid) and a PDB code of 1ECE. This construct was codon optimized for E. coli.
Literature data for this part can be found http://aem.asm.org/content/76/19/6360.full
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]