Difference between revisions of "Part:BBa K2081000:Design"

 
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===Design Notes===
 
===Design Notes===
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We had to take into account what restriction enzymes could be used in order to further engineer the composed plasmid. We used Xba1, EcoR 1, Spe! and Pst1 because they are compatible with the rest of the plasmids in the registry.
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We had to take into account what restriction enzymes could be used in order to further engineer the composed plasmid.
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===Source===
 
===Source===
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TBG complete sequence for plasmid insertion (with Ecor1, Xba1, T4, LacO, RBS: BBA J61110,terminator:BBA_B0015, SPE1, PST)
 
TBG complete sequence for plasmid insertion (with Ecor1, Xba1, T4, LacO, RBS: BBA J61110,terminator:BBA_B0015, SPE1, PST)
  
−
GAATTCTCTAGATAATACGACTCACTATAGGGTGGAATTGTGAGCGGATAACAATTTCTAGAGAAAGAGGCGAATTACTAGATGgagatgcagg tggattcttg ggcatttgct attaacttcc ttccaaaatg tcaccattcc tgtatctggt tctcttggta cttgggcttc atgctacaat ccactgtgca tcacctgaag gcaaagtaac agcctgccat tcatcccaac caaatgccac tctctacaag atgtcatcca ttaatgctga ctttgcattc aatctgtacc ggaggttcac tgtggagacc ccagataaga acatcttctt ttcccctgtg agcatttctg cagctttggt tatgctttcc tttggggcct gctgcagcac ccaaactgag attgtggaga ccttggggtt caacctcaca gacactccaa tggtagagat ccagcatggc ttccagcatc tgatctgttc actgaatttt ccaaagaagg aactggaatt gcagatagga aatgccctct tcattggcaa gcatctgaaa ccactggcaa agttcttgaa tgatgtcaag accctctatg agactgaagt cttttctacc gacttctcca acatttctgc agccaagcag gagattaaca gtcatgtgga gatgcaaacc aaagggaaag ttgtgggtct aattcaagac ctcaagccaa acaccatcat ggtcttagtg aactatattc actttaaagc ccagtgggca aatccttttg atccatccaa gacagaagac agttccagct tcttaataga caagaccacc actgttcaag tgcccatgat gcaccagatg gaacaatact atcacctagt ggatatggaa ttgaactgca cagttctgca aatggactac agcaagaatg ctctggcact ctttgttctt cccaaggagg gacagatgga gtcagtggaa gctgccatgt catctaaaac actgaagaag tggaaccgct tactacagaa gggatgggtt gacttgtttg ttccaaagtt ttccatttct gccacatatg accttggagc cacacttttg aagatgggca ttcagcatgc ctattctgaa aatgctgatt tttctggact cacagaggac aatggtctga aactttccaa tgctgcccat aaggctgtgc tgcacattgg tgaaaaggga actgaagctg cagctgtccc tgaagttgaa ctttcggatc agcctgaaaa cactttccta caccctatta tccaaattga tagatctttc atgttgttga ttttggagag aagcacaagg agtattctct ttctagggaa agttgtgaac ccaacggaag cgtagttggg aaaaaggcca ttggctaatt gcacgtgtgt attgcaatgg gaaataaata aataatatag cctggtgtga ttgatgtgag cttggacttg cattccctta tgatgggatg aagattgaac cctggctgaa ctttgttggc tgtggaagag gccaatccta tggcagagca ttcagaatgt caatgagtaa ttcattatta tccaaagcat aggaaggctc tatgtttgta tatttctctt tgtcagaata cccctcaact catttgctct aataaatttg actgggttga aaaattaaaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggtgggctttctgcgtttataACTAGTCTGCAG
+
gaattctcta gataatacga ctcactatag ggtggaattg tgagcggata acaatttcta gagaaagagg cgaattacta gatggagatg caggtggatt cttgggcatt tgct attaacttcc ttccaaaatg tcaccattcc tgtatctggt tctcttggta cttgggcttc atgctacaat ccactgtgca tcacctgaag gcaaagtaac agcctgccat tcatcccaac caaatgccac tctctacaag atgtcatcca ttaatgctga ctttgcattc aatctgtacc ggaggttcac tgtggagacc ccagataaga acatcttctt ttcccctgtg agcatttctg cagctttggt tatgctttcc tttggggcct gctgcagcac ccaaactgag attgtggaga ccttggggtt caacctcaca gacactccaa tggtagagat ccagcatggc ttccagcatc tgatctgttc actgaatttt ccaaagaagg aactggaatt gcagatagga aatgccctct tcattggcaa gcatctgaaa ccactggcaa agttcttgaa tgatgtcaag accctctatg agactgaagt cttttctacc gacttctcca acatttctgc agccaagcag gagattaaca gtcatgtgga gatgcaaacc aaagggaaag ttgtgggtct aattcaagac ctcaagccaa acaccatcat ggtcttagtg aactatattc actttaaagc ccagtgggca aatccttttg atccatccaa gacagaagac agttccagct tcttaataga caagaccacc actgttcaag tgcccatgat gcaccagatg gaacaatact atcacctagt ggatatggaa ttgaactgca cagttctgca aatggactac agcaagaatg ctctggcact ctttgttctt cccaaggagg gacagatgga gtcagtggaa gctgccatgt catctaaaac actgaagaag tggaaccgct tactacagaa gggatgggtt gacttgtttg ttccaaagtt ttccatttct gccacatatg accttggagc cacacttttg aagatgggca ttcagcatgc ctattctgaa aatgctgatt tttctggact cacagaggac aatggtctga aactttccaa tgctgcccat aaggctgtgc tgcacattgg tgaaaaggga actgaagctg cagctgtccc tgaagttgaa ctttcggatc agcctgaaaa cactttccta caccctatta tccaaattga tagatctttc atgttgttga ttttggagag aagcacaagg agtattctct ttctagggaa agttgtgaac ccaacggaag cgtagttggg aaaaaggcca ttggctaatt gcacgtgtgt attgcaatgg gaaataaata aataatatag cctggtgtga ttgatgtgag cttggacttg cattccctta tgatgggatg aagattgaac cctggctgaa ctttgttggc tgtggaagag gccaatccta tggcagagca ttcagaatgt caatgagtaa ttcattatta tccaaagcat aggaaggctc tatgtttgta tatttctctt tgtcagaata cccctcaact catttgctct aataaatttg actgggttga aaaattaaaa ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcggtg ggctttctgc gtttataact agtctgcag
  
 
===References===
 
===References===
 +
National Center of Biotechnology Information. (2015). Homo sapiens serpin family A member 7 (SERPINA7), mRNA - nucleotide. Retrieved October 15, 2016, from NCBI, https://www.ncbi.nlm.nih.gov/nuccore/NM_000354.5

Latest revision as of 23:54, 29 October 2016


Human thyroxine-binding globulin


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 45
    Illegal PstI site found at 340
    Illegal PstI site found at 374
    Illegal PstI site found at 619
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 340
    Illegal PstI site found at 374
    Illegal PstI site found at 619
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 45
    Illegal PstI site found at 340
    Illegal PstI site found at 374
    Illegal PstI site found at 619
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 45
    Illegal PstI site found at 340
    Illegal PstI site found at 374
    Illegal PstI site found at 619
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 297
    Illegal BsaI.rc site found at 399


Design Notes

We had to take into account what restriction enzymes could be used in order to further engineer the composed plasmid.

Source

TBG complete sequence for plasmid insertion (with Ecor1, Xba1, T4, LacO, RBS: BBA J61110,terminator:BBA_B0015, SPE1, PST)

gaattctcta gataatacga ctcactatag ggtggaattg tgagcggata acaatttcta gagaaagagg cgaattacta gatggagatg caggtggatt cttgggcatt tgct attaacttcc ttccaaaatg tcaccattcc tgtatctggt tctcttggta cttgggcttc atgctacaat ccactgtgca tcacctgaag gcaaagtaac agcctgccat tcatcccaac caaatgccac tctctacaag atgtcatcca ttaatgctga ctttgcattc aatctgtacc ggaggttcac tgtggagacc ccagataaga acatcttctt ttcccctgtg agcatttctg cagctttggt tatgctttcc tttggggcct gctgcagcac ccaaactgag attgtggaga ccttggggtt caacctcaca gacactccaa tggtagagat ccagcatggc ttccagcatc tgatctgttc actgaatttt ccaaagaagg aactggaatt gcagatagga aatgccctct tcattggcaa gcatctgaaa ccactggcaa agttcttgaa tgatgtcaag accctctatg agactgaagt cttttctacc gacttctcca acatttctgc agccaagcag gagattaaca gtcatgtgga gatgcaaacc aaagggaaag ttgtgggtct aattcaagac ctcaagccaa acaccatcat ggtcttagtg aactatattc actttaaagc ccagtgggca aatccttttg atccatccaa gacagaagac agttccagct tcttaataga caagaccacc actgttcaag tgcccatgat gcaccagatg gaacaatact atcacctagt ggatatggaa ttgaactgca cagttctgca aatggactac agcaagaatg ctctggcact ctttgttctt cccaaggagg gacagatgga gtcagtggaa gctgccatgt catctaaaac actgaagaag tggaaccgct tactacagaa gggatgggtt gacttgtttg ttccaaagtt ttccatttct gccacatatg accttggagc cacacttttg aagatgggca ttcagcatgc ctattctgaa aatgctgatt tttctggact cacagaggac aatggtctga aactttccaa tgctgcccat aaggctgtgc tgcacattgg tgaaaaggga actgaagctg cagctgtccc tgaagttgaa ctttcggatc agcctgaaaa cactttccta caccctatta tccaaattga tagatctttc atgttgttga ttttggagag aagcacaagg agtattctct ttctagggaa agttgtgaac ccaacggaag cgtagttggg aaaaaggcca ttggctaatt gcacgtgtgt attgcaatgg gaaataaata aataatatag cctggtgtga ttgatgtgag cttggacttg cattccctta tgatgggatg aagattgaac cctggctgaa ctttgttggc tgtggaagag gccaatccta tggcagagca ttcagaatgt caatgagtaa ttcattatta tccaaagcat aggaaggctc tatgtttgta tatttctctt tgtcagaata cccctcaact catttgctct aataaatttg actgggttga aaaattaaaa ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcggtg ggctttctgc gtttataact agtctgcag

References

National Center of Biotechnology Information. (2015). Homo sapiens serpin family A member 7 (SERPINA7), mRNA - nucleotide. Retrieved October 15, 2016, from NCBI, https://www.ncbi.nlm.nih.gov/nuccore/NM_000354.5