Difference between revisions of "Part:BBa K2179003"

Latest revision as of 23:48, 29 October 2016

Translational Unit for Chlorite Dismutase Cld(-SP)

Chlorite Dismutase is an enzyme which catalyzes the reduction of chlorite ions to oxygen gas and chloride ions. Cld(-SP) is a translational unit which lacks Chlorite Dismutase original putative signal peptide, and also has a polyhistidine tag appended on it for the purpose of protein purification. The activity of this enzyme can be observed as bubbling of the liquid culture when transformed cells are combined with compounds containing the chlorite oxyanion.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 807
Illegal BsaI.rc site found at 6

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 <h5>Translational Unit for Chlorite Dismutase Cld(-SP)</h5>
 <p>
-Chlorite Dismutase is an enzyme which catalyzes the reduction of chlorite ions to oxygen gas and chloride ions. Cld(-SP) is a translational unit which lacks the original Chlorite Dismutase gene's putative signal peptide, and also has a polyhistidine tag appended on it for the purpose of protein purification. The activity of this enzyme can be observed as bubbling of a liquid culture when transformed cells are combined with compounds containing the chlorite oxyanion.
+Chlorite Dismutase is an enzyme which catalyzes the reduction of chlorite ions to oxygen gas and chloride ions. Cld(-SP) is a translational unit which lacks Chlorite Dismutase original putative signal peptide, and also has a polyhistidine tag appended on it for the purpose of protein purification. The activity of this enzyme can be observed as bubbling of the liquid culture when transformed cells are combined with compounds containing the chlorite oxyanion.
 </p>
 <p>
 <partinfo>BBa_K2179003 SequenceAndFeatures</partinfo>