Difference between revisions of "Part:BBa K2179003"
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<h5>Translational Unit for Chlorite Dismutase</h5> | <h5>Translational Unit for Chlorite Dismutase</h5> | ||
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Chlorite Dismutase is an enzyme which catalyzes the reduction of chlorite ions to oxygen gas and chloride ions. Cld(-SP) is a translational unit which lacks the original Chlorite Dismutase gene's putative signal peptide, and also has a polyhistidine tag appended on it for the purpose of protein purification. The activity of this enzyme can be observed as bubbling of a liquid culture when transformed cells are combined with compounds containing the chlorite oxyanion. | Chlorite Dismutase is an enzyme which catalyzes the reduction of chlorite ions to oxygen gas and chloride ions. Cld(-SP) is a translational unit which lacks the original Chlorite Dismutase gene's putative signal peptide, and also has a polyhistidine tag appended on it for the purpose of protein purification. The activity of this enzyme can be observed as bubbling of a liquid culture when transformed cells are combined with compounds containing the chlorite oxyanion. | ||
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Revision as of 23:40, 29 October 2016
Translational Unit for Chlorite Dismutase
Chlorite Dismutase CDS+RBS from I. dechloratans with His-tag
Chlorite Dismutase is an enzyme which catalyzes the reduction of chlorite ions to oxygen gas and chloride ions. Cld(-SP) is a translational unit which lacks the original Chlorite Dismutase gene's putative signal peptide, and also has a polyhistidine tag appended on it for the purpose of protein purification. The activity of this enzyme can be observed as bubbling of a liquid culture when transformed cells are combined with compounds containing the chlorite oxyanion.