Difference between revisions of "Part:BBa K1110003:Experience"

(Applications of BBa_K1110003)
(Applications of BBa_K1110003)
 
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[[File:T--Georgia_State--Mambalgin_Ppastoris_Coomassie.png|none|500px]]
 
[[File:T--Georgia_State--Mambalgin_Ppastoris_Coomassie.png|none|500px]]
 
'''Figure 1: Coomassie Gel Stain of Mambalgin-1 in Pichia pastoris Protein Expression.'''
 
'''Figure 1: Coomassie Gel Stain of Mambalgin-1 in Pichia pastoris Protein Expression.'''
"Coomassie stain of SDS-PAGE run at 100 volts for 30 minutes"
+
''Coomassie stain of SDS-PAGE run at 100 volts for 30 minutes.''
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 23:19, 29 October 2016


Applications of BBa_K1110003

Mambalgin-1 was expressed in Pichia pastoris and confirmed with colony PCR. Expression was shown with a western blot and coomassie stain. The coomassie stain resulted in a band near 9kDa, which was the expected band size. Additionally, further purification of the supernatant was not needed after initial centrifugation. Thus, protein expression of Mambalgin-1 in P. pastoris required a minimal amount of steps.

T--Georgia State--Pichia Mambalgin1 Coomassie.png


T--Georgia State--Mambalgin Ppastoris Coomassie.png

Figure 1: Coomassie Gel Stain of Mambalgin-1 in Pichia pastoris Protein Expression. Coomassie stain of SDS-PAGE run at 100 volts for 30 minutes.

User Reviews

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