Difference between revisions of "Part:BBa K1110003:Experience"

Latest revision as of 23:19, 29 October 2016

Applications of BBa_K1110003

Mambalgin-1 was expressed in Pichia pastoris and confirmed with colony PCR. Expression was shown with a western blot and coomassie stain. The coomassie stain resulted in a band near 9kDa, which was the expected band size. Additionally, further purification of the supernatant was not needed after initial centrifugation. Thus, protein expression of Mambalgin-1 in P. pastoris required a minimal amount of steps.

T--Georgia State--Pichia Mambalgin1 Coomassie.png

T--Georgia State--Mambalgin Ppastoris Coomassie.png

Figure 1: Coomassie Gel Stain of Mambalgin-1 in Pichia pastoris Protein Expression. Coomassie stain of SDS-PAGE run at 100 volts for 30 minutes.

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UNIQ3236f5dc56b84ee0-partinfo-00000000-QINU UNIQ3236f5dc56b84ee0-partinfo-00000001-QINU

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 __NOTOC__
-Mambalgin-1 was expressed in Pichia pastoris and confirmed with colony PCR. Expression was shown with a western blot and coomassie stain. The coomassie stain resulted in a band near 9kDa, which was the expected band size.
 ===Applications of BBa_K1110003===
+Mambalgin-1 was expressed in Pichia pastoris and confirmed with colony PCR. Expression was shown with a western blot and coomassie stain. The coomassie stain resulted in a band near 9kDa, which was the expected band size. Additionally, further purification of the supernatant was not needed after initial centrifugation. Thus, protein expression of Mambalgin-1 in P. pastoris required a minimal amount of steps.
+[[File:T--Georgia_State--Pichia_Mambalgin1_Coomassie.png|none|600px]]
+[[File:T--Georgia_State--Mambalgin_Ppastoris_Coomassie.png|none|500px]]
+'''Figure 1: Coomassie Gel Stain of Mambalgin-1 in Pichia pastoris Protein Expression.'''
+''Coomassie stain of SDS-PAGE run at 100 volts for 30 minutes.''
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