Difference between revisions of "Part:BBa K1110003:Experience"
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'''Figure 1: Coomassie Gel Stain of Mambalgin-1 in Pichia pastoris Protein Expression.''' | '''Figure 1: Coomassie Gel Stain of Mambalgin-1 in Pichia pastoris Protein Expression.''' | ||
"Coomassie stain of SDS-PAGE run at 100 volts for 30 minutes" | "Coomassie stain of SDS-PAGE run at 100 volts for 30 minutes" | ||
Revision as of 23:05, 29 October 2016
Applications of BBa_K1110003
Mambalgin-1 was expressed in Pichia pastoris and confirmed with colony PCR. Expression was shown with a western blot and coomassie stain. The coomassie stain resulted in a band near 9kDa, which was the expected band size.
Figure 1: Coomassie Gel Stain of Mambalgin-1 in Pichia pastoris Protein Expression. "Coomassie stain of SDS-PAGE run at 100 volts for 30 minutes"
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