Difference between revisions of "Part:BBa K2092002:Design"

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===Design Notes===
 
===Design Notes===
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To add the missing XbaI and SpeI restriction sites, the following two primers were designed:
+
XbaI and SpeI restriction sites were missing in the original BioBrick by 2011 iGEM DTU-Denmark.  To add the following sites, two primers were designed:
 +
 
 
alcA forward primer = 5'- CGG AAT TCG CGT CTA GAA CGT CGC TCT CCC CGA TGA C -3'  
 
alcA forward primer = 5'- CGG AAT TCG CGT CTA GAA CGT CGC TCT CCC CGA TGA C -3'  
 +
 
alcA reverse primer =  
 
alcA reverse primer =  
 
5'- AAC TGC AGA AAC CAA TGC ATT GGA CTA GTT TTT GAG GCG AGG TGA TAG GAT TGG -3'
 
5'- AAC TGC AGA AAC CAA TGC ATT GGA CTA GTT TTT GAG GCG AGG TGA TAG GAT TGG -3'
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−
 
−
 
  
 
===Source===
 
===Source===
  
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Aspergillus nidulans, sequence found through
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2011 iGEM DTU-Denmark 2
  
 
===References===
 
===References===

Latest revision as of 19:25, 29 October 2016


PalcA, improved alcR inducible promoter from A. nidulans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 274
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

XbaI and SpeI restriction sites were missing in the original BioBrick by 2011 iGEM DTU-Denmark. To add the following sites, two primers were designed:

alcA forward primer = 5'- CGG AAT TCG CGT CTA GAA CGT CGC TCT CCC CGA TGA C -3'

alcA reverse primer = 5'- AAC TGC AGA AAC CAA TGC ATT GGA CTA GTT TTT GAG GCG AGG TGA TAG GAT TGG -3'

Source

2011 iGEM DTU-Denmark 2

References