Difference between revisions of "Part:BBa K2074032"
 
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<partinfo>BBa_K2074032 short</partinfo>
 
<partinfo>BBa_K2074032 short</partinfo>
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https://static.igem.org/mediawiki/igem.org/thumb/9/9f/T--FAFU-CHINA--partsclones.png/800px-T--FAFU-CHINA--partsclones.png
  
 
Cry10Aa and Cyt2 are protein from Bacillus thuringiensis (Bt),which have toxicity to the larave of mosquito.We used 2A system to express two protein at the same time in Chlamydomonas reinhardti. These two proteins express at the same time can enhance the  toxicity to the larvae of mosquito.
 
Cry10Aa and Cyt2 are protein from Bacillus thuringiensis (Bt),which have toxicity to the larave of mosquito.We used 2A system to express two protein at the same time in Chlamydomonas reinhardti. These two proteins express at the same time can enhance the  toxicity to the larvae of mosquito.
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[[File:2016FAFUCHINADevice5.png|800px|thumb|center|'''Fig.1''' Cry10Aa and Cyt2 Toxins Group]]
 
[[File:2016FAFUCHINADevice5.png|800px|thumb|center|'''Fig.1''' Cry10Aa and Cyt2 Toxins Group]]
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[[File:T--FAFU-CHINA--11+2.png|600px|thumb|center|'''Fig.2''' The gel electrophoresis result of Cry11Aa+FMDV 2A+Cyt2]]
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[[File:T--FAFU-CHINA--Result3.png|600px|thumb|center]]
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 17:43, 29 October 2016


Cry11Aa(Codon optimization)+Extended FMDV 2A+Cyt2(Codon optimization) 800px-T--FAFU-CHINA--partsclones.png

Cry10Aa and Cyt2 are protein from Bacillus thuringiensis (Bt),which have toxicity to the larave of mosquito.We used 2A system to express two protein at the same time in Chlamydomonas reinhardti. These two proteins express at the same time can enhance the toxicity to the larvae of mosquito.

Fig.1 Cry10Aa and Cyt2 Toxins Group
Fig.2 The gel electrophoresis result of Cry11Aa+FMDV 2A+Cyt2
T--FAFU-CHINA--Result3.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2195
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 517
    Illegal NgoMIV site found at 1681
    Illegal NgoMIV site found at 2029
    Illegal NgoMIV site found at 2329
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 49
    Illegal BsaI.rc site found at 244
    Illegal BsaI.rc site found at 1459
    Illegal SapI.rc site found at 1440