Difference between revisions of "Part:BBa K2074034"

Latest revision as of 17:42, 29 October 2016

Cry11Aa(Codon optimization)+2A+EGFP(Codon optimization)

We use 2A system to link cry11Aa and EGFP then transferred the gene into plasmid ,and transformed the recombinant plasmid into Chlamydomonas reinhardti to express protein .

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Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 517
Illegal NgoMIV site found at 1681
Illegal NgoMIV site found at 2029
Illegal NgoMIV site found at 2740
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 49
Illegal BsaI.rc site found at 244
Illegal BsaI.rc site found at 1459
Illegal SapI.rc site found at 1440

@@ Line 4: / Line 4: @@
 https://static.igem.org/mediawiki/igem.org/thumb/9/9f/T--FAFU-CHINA--partsclones.png/800px-T--FAFU-CHINA--partsclones.png
-we use 2A system to link cry11Aa and EGFP then transferred the gene into plasmid ,and transformed the recombinant plasmid into Chlamydomonas reinhardti to express protein .
+We use 2A system to link cry11Aa and EGFP then transferred the gene into plasmid ,and transformed the recombinant plasmid into Chlamydomonas reinhardti to express protein .
+[[File:Confocalcry11.png|600px|thumb|center|'''']]
+[[File:T--FAFU-CHINA--Result3.png|600px|thumb|center]]
+[[File:K2074034.png|600px|thumb|center]]
 <!-- Add more about the biology of this part here
-[[File:T--FAFU-CHINA--4+E.png|600px|thumb|center|'''Figure.1''' The gel electrophoresis result of Cry11Aa+FMDV 2A+EGFP]]
 ===Usage and Biology===