Difference between revisions of "Part:BBa K1952010"
 
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Hydrazine Synthase subunit gamma (Kust-2859) with strong RBS under lactose-inducible promoter, followed by LacZ-alpha reporter gene (protein fusion).
 
Hydrazine Synthase subunit gamma (Kust-2859) with strong RBS under lactose-inducible promoter, followed by LacZ-alpha reporter gene (protein fusion).
 +
 +
Used to verify proper expression of Hydrazine Synthase gamma subunit in E. coli (demonstrate expression by blue color of bacteria in the presence of IPTG and X-gal.)
 +
 +
Preliminary test indicates that reporter gene is expressed, indicating that the fusion protein is expressed, active, and non-toxic to the cells.
  
 
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Latest revision as of 15:49, 29 October 2016


Hydrazine Synthase subunit gamma (Kust-2859) with LacZ reporter

Hydrazine Synthase subunit gamma (Kust-2859) with strong RBS under lactose-inducible promoter, followed by LacZ-alpha reporter gene (protein fusion).

Used to verify proper expression of Hydrazine Synthase gamma subunit in E. coli (demonstrate expression by blue color of bacteria in the presence of IPTG and X-gal.)

Preliminary test indicates that reporter gene is expressed, indicating that the fusion protein is expressed, active, and non-toxic to the cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 445
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 407
    Illegal XhoI site found at 1474
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 568
    Illegal AgeI site found at 1461
  • 1000
    COMPATIBLE WITH RFC[1000]