Difference between revisions of "Part:BBa K1952010"

Latest revision as of 15:49, 29 October 2016

Hydrazine Synthase subunit gamma (Kust-2859) with LacZ reporter

Hydrazine Synthase subunit gamma (Kust-2859) with strong RBS under lactose-inducible promoter, followed by LacZ-alpha reporter gene (protein fusion).

Used to verify proper expression of Hydrazine Synthase gamma subunit in E. coli (demonstrate expression by blue color of bacteria in the presence of IPTG and X-gal.)

Preliminary test indicates that reporter gene is expressed, indicating that the fusion protein is expressed, active, and non-toxic to the cells.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 445
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 407
Illegal XhoI site found at 1474
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 568
Illegal AgeI site found at 1461
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 Hydrazine Synthase subunit gamma (Kust-2859) with strong RBS under lactose-inducible promoter, followed by LacZ-alpha reporter gene (protein fusion).
+Used to verify proper expression of Hydrazine Synthase gamma subunit in E. coli (demonstrate expression by blue color of bacteria in the presence of IPTG and X-gal.)
+Preliminary test indicates that reporter gene is expressed, indicating that the fusion protein is expressed, active, and non-toxic to the cells.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+Used to verify proper expression of Hydrazine Synthase gamma subunit in E. coli (demonstrate expression by blue color of bacteria in the presence of IPTG and X-gal.
 <!-- -->