Difference between revisions of "Part:BBa K1893002"

Latest revision as of 15:03, 29 October 2016

Rhl receiver (RhlR+pRhl)

The Rhl receiver device consists of a constitutively expressed RhlR transcription regulator (BBa_C0171) upstream of a cognate pRhl promoter (BBa_R0071). RhlR is activated in the presence of a C4-AHL signal and binds to pRhl to initiate transcription of a downstream coding sequence.

Usage and biology

The Rhl receiver is based on the Rhl quorum-sensing system from Pseudomonas aeruginosa. Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression.

In this case, the constitutively expressed RhlR transcriptional regulator (BBa_C0171) is activated by the binding of C4-AHL, an AHL quorum signal. The activated RhlR regulator binds to the RhlR-inducible promoter, pRhl, to initiate transcription of a downstream gene.

Characterisation

We characterised the activation range of this device using a GFP reporter. The results of our characterisation experiments can be found here (BBa_K1893003).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 301
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 776

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 <partinfo>BBa_K1893002 short</partinfo>
-The gene encoding the transcriptional activator RhlR is downstream a constitutive Anderson promoter, j23101. RhlR is activated by C4-HSL. Included in the construct is the RhlR-activated promoter pRhl.
 The Rhl receiver device consists of a constitutively expressed RhlR transcription regulator [https://parts.igem.org/Part:BBa_C0171 (BBa_C0171)] upstream of a cognate pRhl promoter [https://parts.igem.org/Part:BBa_R0071 (BBa_R0071)]. RhlR is activated in the presence of a C4-AHL signal and binds to pRhl to initiate transcription of a downstream coding sequence.