Difference between revisions of "Part:BBa J45200"

Revision as of 17:14, 10 October 2007

Banana odor generator

Protein generator producing the protein ATF1 under control of the LacI repressor. Thus, in a LacI^- E. coli strain, ATF1 is continually produced. ATFI encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols (including isoamyl acetate from isoamyl alcohol). This part is almost identical to BBa_J45220 except that it uses the B0030 RBS (instead of B0032 as in BBa_J45220). The Banana Generating Device (BGD) function thus far has been to convert isoamyl alcohol into isoamyl acetate (a banana-scented compound).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 182
Illegal BamHI site found at 1509
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 609
Illegal SapI.rc site found at 1617

@@ Line 2: / Line 2: @@
 <partinfo>BBa_J45200 short</partinfo>
-Protein generator producing the protein ATF1 under control of the LacI repressor.  Thus, in a LacI<sup>-</sup> ''E. coli'' strain, ATF1 is continually produced.  ATFI encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols (including isoamyl acetate from isoamyl alcohol).  This part is almost identical to <partinfo>J45220</partinfo>  except that it uses the B0030 RBS (instead of B0032 as is in <partinfo>J45220</partinfo>).  The Banana Generating Device (BGD) function thus far has been to convert isoamyl alcohol into isoamyl acetate (a banana-scented compound).
+Protein generator producing the protein ATF1 under control of the LacI repressor.  Thus, in a LacI<sup>-</sup> ''E. coli'' strain, ATF1 is continually produced.  ATFI encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols (including isoamyl acetate from isoamyl alcohol).  This part is almost identical to <partinfo>J45220</partinfo>  except that it uses the B0030 RBS (instead of B0032 as in <partinfo>J45220</partinfo>).  The Banana Generating Device (BGD) function thus far has been to convert isoamyl alcohol into isoamyl acetate (a banana-scented compound).
 [[Image:J45200.jpg]]