Difference between revisions of "Part:BBa K1915003:Experience"
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Colony PCR of transformed inducible lac promoter + LTNF15 in PSB1C3. Lanes 1,2,3,4,5,7,9,10 gave us bands around 500 basepairs which is what was expected for the PCR reaction using our VF2 and VR primers. See the figure 1 above. | Colony PCR of transformed inducible lac promoter + LTNF15 in PSB1C3. Lanes 1,2,3,4,5,7,9,10 gave us bands around 500 basepairs which is what was expected for the PCR reaction using our VF2 and VR primers. See the figure 1 above. | ||
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Revision as of 05:08, 29 October 2016
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Applications of BBa_K1915003
Figure 1. Gel of Inducible Lac Promoter+LTNF-15. The above is a gel of the colony PCR of the inducible lac promoter+LTNF-15.
Colony PCR of transformed inducible lac promoter + LTNF15 in PSB1C3. Lanes 1,2,3,4,5,7,9,10 gave us bands around 500 basepairs which is what was expected for the PCR reaction using our VF2 and VR primers. See the figure 1 above.
Figure 2. Gel of Inducible Lac Promoter+LTNF-15. The above is a gel of the inducible lac promoter+LTN-15 digested with BamHI.
LTNF has a BamHI restriction site that we utilized to help diagnose if the inducible lac promoter + LTNF15 was in the pSB1C3 vector. The first lane contains the plasmid lacking the BamHI restriction site. Each restriction digest was treated the same. Lanes 2,3,4, and 5 have the inducible lac promoter + LTNF15 cut with BamH1. Bands appear around the expected 2289 bp.See the figure 2.
Figure 3. Gel of Inducible Lac Promoter+LTN-15. The above is a gel of the inducible lac promoter+LTN-15 digested with EcoRI and PstI.
A restriction digest of the inducible lac promoter + LTNF15 was done using EcoRI and Pst1. The plasmid pSB1C3 served as a control because we knew that the bands produced would be larger in size than the bands produced from our digest of BBa_ K1915003. In lane 1 the plasmid pSB1C3 with RFP was digested with EcoRI and in lane 2 the plasmid pSB1C3 with RFP was digested with EcoRI and PstI. Lanes 4,6,8, and 10 show the inducible lac promoter + LTNF15 around the expected size of 260 bp.See the figure 3 above.
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