Difference between revisions of "Part:BBa K2066037"
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| − | + | This is the pACT-Tet sequence from Amit et al. 2011 “Building Synthetic Enhancers from the Ground Up” paper on the UNS standard. This part consists of the endogenous nitrogen regulatory promoter, the NRII mutant BBa_K2066112 and tetR. This is meant for use in Tet based synthetic enhancer systems, such as the [http://2016.igem.org/Team:William_and_Mary/Synthetic_Enhancer William and Mary 2016] | |
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Latest revision as of 04:50, 29 October 2016
pACT-Tet on UNS Standard
This is the pACT-Tet sequence from Amit et al. 2011 “Building Synthetic Enhancers from the Ground Up” paper on the UNS standard. This part consists of the endogenous nitrogen regulatory promoter, the NRII mutant BBa_K2066112 and tetR. This is meant for use in Tet based synthetic enhancer systems, such as the [http://2016.igem.org/Team:William_and_Mary/Synthetic_Enhancer William and Mary 2016]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 969
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 2343
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]