Difference between revisions of "Part:BBa K1893014"
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<partinfo>BBa_K1893014 short</partinfo> | <partinfo>BBa_K1893014 short</partinfo> | ||
| − | + | Anti-STAR is the reverse complement of STAR [https://parts.igem.org/Part:BBa_K1893013 (BBa_K1893013)]. It is identical to part of the STAR target sequence (BBa_K1893031), but much shorter in length and binds to STAR with greater affinity. | |
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| + | ===Usage and biology=== | ||
| + | The anti-STAR DNA sequence is identical to part of the 5’ end of the STAR target DNA sequence, but shorter in length. Anti-STAR was designed as an inhibitory RNA sequence for STAR. When transcribed, it produces a RNA product that is fully complementary to the transcribed antisense-STAR product. Anti-STAR has a higher affinity for STAR than the STAR target sequence, which allows it to sequester STAR and prevents it from binding to the STAR target sequence to activate transcription of a downstream coding sequence. | ||
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| + | Anti-STAR was designed by using RNAstructure, a software tool that allowed us to predict a complementary RNA sequence to STAR that binds with higher affinity to STAR than it does to the full STAR target sequence. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1893014 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1893014 SequenceAndFeatures</partinfo> | ||
Latest revision as of 04:29, 29 October 2016
anti-STAR
Anti-STAR is the reverse complement of STAR (BBa_K1893013). It is identical to part of the STAR target sequence (BBa_K1893031), but much shorter in length and binds to STAR with greater affinity.
Usage and biology
The anti-STAR DNA sequence is identical to part of the 5’ end of the STAR target DNA sequence, but shorter in length. Anti-STAR was designed as an inhibitory RNA sequence for STAR. When transcribed, it produces a RNA product that is fully complementary to the transcribed antisense-STAR product. Anti-STAR has a higher affinity for STAR than the STAR target sequence, which allows it to sequester STAR and prevents it from binding to the STAR target sequence to activate transcription of a downstream coding sequence.
Anti-STAR was designed by using RNAstructure, a software tool that allowed us to predict a complementary RNA sequence to STAR that binds with higher affinity to STAR than it does to the full STAR target sequence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]