Difference between revisions of "Part:BBa K2066033"
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<partinfo>BBa_K2066033 short</partinfo> | <partinfo>BBa_K2066033 short</partinfo> | ||
− | This part is used to test the induction of the pBad promoter. Its insert is the I0050 functional RFP cassette from the 2016 Distribution Kit, BBa_K577882, but flanked by UNS2 and UNS3. | + | This part is used to test the induction of the pBad promoter. Its insert is the I0050 functional RFP cassette from the 2016 Distribution Kit, BBa_K577882, but flanked by UNS2 (BBa_K2066018) and UNS3 (BBa_K2066019) to put the insert onto the UNS backbone standard developed by William and Mary iGEM 2016. |
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Latest revision as of 04:20, 29 October 2016
pBad - RFP on UNS
This part is used to test the induction of the pBad promoter. Its insert is the I0050 functional RFP cassette from the 2016 Distribution Kit, BBa_K577882, but flanked by UNS2 (BBa_K2066018) and UNS3 (BBa_K2066019) to put the insert onto the UNS backbone standard developed by William and Mary iGEM 2016.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1287
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1227
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1019
Illegal AgeI site found at 1873
Illegal AgeI site found at 1985 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1001