Difference between revisions of "Part:BBa K2066033"

 
 
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<partinfo>BBa_K2066033 short</partinfo>
 
<partinfo>BBa_K2066033 short</partinfo>
  
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This part is used to test the induction of the pBad promoter. Its insert is the I0050 functional RFP cassette from the 2016 Distribution Kit, K577882, but flanked by UNS2 and UNS3.
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This part is used to test the induction of the pBad promoter. Its insert is the I0050 functional RFP cassette from the 2016 Distribution Kit, BBa_K577882, but flanked by UNS2 (BBa_K2066018) and UNS3 (BBa_K2066019) to put the insert onto the UNS backbone standard developed by William and Mary iGEM 2016.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 04:20, 29 October 2016


pBad - RFP on UNS

This part is used to test the induction of the pBad promoter. Its insert is the I0050 functional RFP cassette from the 2016 Distribution Kit, BBa_K577882, but flanked by UNS2 (BBa_K2066018) and UNS3 (BBa_K2066019) to put the insert onto the UNS backbone standard developed by William and Mary iGEM 2016.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1287
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1019
    Illegal AgeI site found at 1873
    Illegal AgeI site found at 1985
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1001