Difference between revisions of "Part:BBa K2165000:Design"

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===Source===
 
===Source===
  
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​VioC​ ​is​ ​originally​ ​from​ ​the​ violacein​ ​metabolic pathway​ ​in​ ​Chromobacterium​ ​Violaceum​, a type of Proteobacteria.  The original vioC sequence for the bacteria chassis was submitted by the Alberta team in 2013 as K1161003 (https://parts.igem.org/wiki/index.php?title=Part:BBa_K1161003) and has been codon-optimized for use in yeast in this part.
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The CDS contained in this BioBrick was designed by running the VioC gene sent to the University of Washington by the [http://dueberlab.berkeley.edu/ Dueber Laboratory] at University of California-Berkley through IDT's Codon Optimization Tool for S. cerevisiae. A biobrick standard assembly prefix and suffix was added before the it was ordered as a geneblock through IDT.
  
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===References===
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===Characterization===
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[[File:BBa_K2165000_Violcultures.png|thumb|left|200px|Constituatively active VioABCDE yeast in synthetic media, along with a positive control (regular yeast) and a negative control (no yeast)]]

Revision as of 04:01, 29 October 2016


Violacein C gene codon-optimized for S. Cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101


Design Notes

The online codon-optimization tool offered by Integrated DNA Technologies (https://www.idtdna.com/CodonOpt) was used to codon-optimize this sequence for use in yeast. Combinations containing RFC[10] illegal restriction sites were actively avoided.

Source

The CDS contained in this BioBrick was designed by running the VioC gene sent to the University of Washington by the [http://dueberlab.berkeley.edu/ Dueber Laboratory] at University of California-Berkley through IDT's Codon Optimization Tool for S. cerevisiae. A biobrick standard assembly prefix and suffix was added before the it was ordered as a geneblock through IDT.

Characterization

Constituatively active VioABCDE yeast in synthetic media, along with a positive control (regular yeast) and a negative control (no yeast)