Difference between revisions of "Part:BBa K1893014"
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<partinfo>BBa_K1893014 short</partinfo>
 
<partinfo>BBa_K1893014 short</partinfo>
  
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Anti-STAR is identical to part of the STAR-target attenuator sequence (pAD1.S5) but much shorter in length. When transcribed, it is fully complementary and thus binds to the STAR-antisense RNA (pAD1.A5) with much higher affinity than the STAR-target sequence.
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Anti-STAR is the reverse complement of STAR [https://parts.igem.org/Part:BBa_K1893013 (BBa_K1893013)]. It is identical to part of the STAR target sequence (BBa_K1893031), but much shorter in length and binds to STAR with greater affinity.
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===Usage and biology===
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The anti-STAR DNA sequence is identical to part of the 5’ end of the STAR target sense DNA sequence, but shorter in length. Anti-STAR was designed as an inhibitory RNA sequence for STAR. When transcribed, it produces a RNA product that is fully complementary to the transcribed antisense-STAR product. Anti-STAR has a higher affinity for STAR than the STAR target sequence, which allows it to sequester STAR and prevents it from binding to the STAR target sequence to activate transcription of a downstream coding sequence.
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Anti-STAR was designed by using RNAstructure, a software tool that allowed us to predict a complementary RNA sequence to STAR that binds with higher affinity to STAR than it does to the full STAR target sequence.
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===Usage and Biology===
 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1893014 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1893014 SequenceAndFeatures</partinfo>

Revision as of 03:24, 29 October 2016


anti-STAR

Anti-STAR is the reverse complement of STAR (BBa_K1893013). It is identical to part of the STAR target sequence (BBa_K1893031), but much shorter in length and binds to STAR with greater affinity.


Usage and biology

The anti-STAR DNA sequence is identical to part of the 5’ end of the STAR target sense DNA sequence, but shorter in length. Anti-STAR was designed as an inhibitory RNA sequence for STAR. When transcribed, it produces a RNA product that is fully complementary to the transcribed antisense-STAR product. Anti-STAR has a higher affinity for STAR than the STAR target sequence, which allows it to sequester STAR and prevents it from binding to the STAR target sequence to activate transcription of a downstream coding sequence.

Anti-STAR was designed by using RNAstructure, a software tool that allowed us to predict a complementary RNA sequence to STAR that binds with higher affinity to STAR than it does to the full STAR target sequence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]