Difference between revisions of "Part:BBa K2066023"

 
 
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<partinfo>BBa_K2066023 short</partinfo>
 
<partinfo>BBa_K2066023 short</partinfo>
  
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This part is essentially R0040 driving expression of GFP. It is K1493504 on a UNS backbone. TetR is a repressor molecule that can bind to the pTET promoter and block the transcription of GFP.  Used this part with BBa_K2066022 to look at aTc induction.  
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This part was created to place the pTet-GFP reporter construct BBa_K1493504 on the UNS backbone standard developed by William and Mary iGEM 2016. It is flanked by UNS 2 (BBa_K2066018) and UNS 3 (BBa_K2066019) sequences.
  
 
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Latest revision as of 02:06, 29 October 2016


pTet GFP + UNS

This part was created to place the pTet-GFP reporter construct BBa_K1493504 on the UNS backbone standard developed by William and Mary iGEM 2016. It is flanked by UNS 2 (BBa_K2066018) and UNS 3 (BBa_K2066019) sequences.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 764