Difference between revisions of "Part:BBa K2066014"

 
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<partinfo>BBa_K2066014 short</partinfo>
 
<partinfo>BBa_K2066014 short</partinfo>
  
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This part is used for 2016 WM iGEM Ribozyme Characterization project.  Lou et. al showed that sequences in the 5' UTR of a gene can affect the input-output relationship (transfer function) of a circuit.  The RiboJ serves as an insulator to generalize the transfer function of a circuit regardless of promoter.  The part is used in combination with  BBa_K2066016 (constitutive Lac Repressor) to look at input-output relationship of IPTG-sfGFP.  
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This part is used for 2016 WM iGEM Ribozyme Characterization project.  Lou et. al showed that sequences in the 5' UTR of a gene can affect the input-output relationship (transfer function) of a circuit.  The RiboJ serves as an insulator to generalize the transfer function of a circuit regardless of output coding sequence.  The part is used in combination with  BBa_K2066016 (constitutive Lac Repressor) to look at input-output relationship of IPTG-sfGFP.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 01:55, 29 October 2016


plLacO-1+RiboJ+sfGFP

This part is used for 2016 WM iGEM Ribozyme Characterization project. Lou et. al showed that sequences in the 5' UTR of a gene can affect the input-output relationship (transfer function) of a circuit. The RiboJ serves as an insulator to generalize the transfer function of a circuit regardless of output coding sequence. The part is used in combination with BBa_K2066016 (constitutive Lac Repressor) to look at input-output relationship of IPTG-sfGFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 224