Difference between revisions of "Part:BBa K2066016"
 
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<partinfo>BBa_K2066016 short</partinfo>
 
<partinfo>BBa_K2066016 short</partinfo>
  
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In order to characterize the transfer function of a promoter with and without RiboJ, it is necessary to induce the activity of that promoter. plLacO-1 and pTac are both repressed by the lac repressor, which in turn is repressed by IPTG— hence IPTG will induce the activity of plLacO-1 and pTac. This part is designed for gBlock synthesis by IDT. It is then meant to be cloned into the plasmid form of WM16_014 or WM16_015 to create WM16_018 and WM16_019, which replicate the plasmids in Lou et al. 2012, “Ribozyme-based insulator parts buffer synthetic circuits from genetic context”.  
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This part codes for constitutive expression of the Lac repressor protein. The part is flanked by the UNS 2 (BBa_K2066018) and UNS 3 (BBa_K2066019) sequences following the UNS backbone standard developed by William and Mary iGEM 2016.  
  
 
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Latest revision as of 01:52, 29 October 2016


Constitutive Lac Repressor on UNS Standard

This part codes for constitutive expression of the Lac repressor protein. The part is flanked by the UNS 2 (BBa_K2066018) and UNS 3 (BBa_K2066019) sequences following the UNS backbone standard developed by William and Mary iGEM 2016.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]