Difference between revisions of "Part:BBa K2066001"

Latest revision as of 01:44, 29 October 2016

UNS Standard Adapter with Red Chromoprotein.

This is a red chromoprotein expression construct on the UNS backbone standard developed by William and Mary iGEM 2016, which includes UNS Sequences developed by Torella et. al 2013 as insert flankers that are interior to the Prefix and Suffix regions. This allows ease of cloning via Gibson Assembly or Golden Gate Assembly.

The purpose of this part is to provide a standard and visible reporter on the UNS standard, for teams to use as a template for obtaining the UNS backbone.

The UNS2 (BBa_K2066018) and UNS3 (BBa_K2066019) sequences are taken from: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 47
Illegal NheI site found at 70
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 The purpose of this part is to provide a standard and visible reporter on the UNS standard, for teams to use as a template for obtaining the UNS backbone.
-The UNS2 and UNS3 sequences are taken from: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.
+The UNS2 (BBa_K2066018) and UNS3 (BBa_K2066019) sequences are taken from: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.
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